For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TCF21 aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: O43680
This product is a recombinant rabbit monoclonal antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab182134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 20 kDa).|
|Flow Cyt||1/130. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling TCF21 with ab182134 at 1/130 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab182134 at 1/70 dilution (Lane 1). Lane 2: Negative control.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
ab182134 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"