Recombinant
RabMAb

Anti-TCF21 antibody [EPR13449] (ab182134)

Overview

  • Product name
    Anti-TCF21 antibody [EPR13449]
    See all TCF21 primary antibodies
  • Description
    Rabbit monoclonal [EPR13449] to TCF21
  • Tested applications
    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TCF21 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: O43680

  • Positive control
    • HeLa and 293 cell lysates; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab182134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 20 kDa).
Flow Cyt 1/130. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/70.

Target

  • Function
    Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. May play a role in the specification or differentiation of one or more subsets of epicardial cell types.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHa23 antibody
    • Capsulin antibody
    • Class A basic helix-loop-helix protein 23 antibody
    • Epicardin antibody
    • POD 1 antibody
    • Pod-1 antibody
    • POD1 antibody
    • Podocyte expressed 1 antibody
    • Podocyte-expressed 1 antibody
    • TCF-21 antibody
    • tcf21 antibody
    • TCF21_HUMAN antibody
    • Transcription factor 21 antibody
    see all

Images

  • All lanes : Anti-TCF21 antibody [EPR13449] (ab182134) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 20 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling TCF21 with ab182134 at 1/130 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab182134 at 1/70 dilution (Lane 1). Lane 2: Negative control.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

ab182134 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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