The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 20 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. May play a role in the specification or differentiation of one or more subsets of epicardial cell types.
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling TCF21 with ab182134 at 1/130 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab182134 at 1/70 dilution (Lane 1). Lane 2: Negative control. Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.