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Anti-TCN2 antibody (ab76638) at 5 µg/ml + immunogen (GST-tagged recombinant full length Human TCN2) at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
Predicted band size : 47 kDa
Observed band size : 73 kDa (why is the actual band size different from the predicted?)
MW of GST tag: 26 kDa
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