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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Overview
Product name
Anti-TCP1 alpha antibody [23c ]
See all TCP1 alpha products (11) ...
Description
Rat monoclonal [23c ] to TCP1 alpha
Specificity
This antibody recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. It does not react with human Hsp60 protein.
Tested applications
WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Rabbit, Hamster, Dog
Immunogen
Purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp 1b gene expressed in E. coli.
Epitope
Ab13501 recognises the COOH group, addition of an alanine (LDDACOOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences.
Positive control
RK13 heat shocked cell lysate.
General notes
Ab13501 binds protein A and is useful for native precipitations of coatomer and CTT.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This Antibody is affinity purified.
Clonality
Monoclonal
Clone number
23c
Isotype
IgG2c
Research areas
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 60 kDa.
IP: Use a concentration of 5 µg/ml
IHC-P: Use at an assay dependent dilution.
Target
Function
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Sequence similarities
Belongs to the TCP-1 chaperonin family.
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome.
Target information above from: UniProt accessionP17987
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI528772 antibody
- c-cpn antibody
- CCT alpha antibody
see all
Database links
- Entrez Gene: 484064 Dog
- Entrez Gene: 21454 Mouse
- Entrez Gene: 100302435 Rabbit
- Entrez Gene: 24818 Rat
- SwissProt: P11983 Mouse
- SwissProt: P28480 Rat
- Unigene: 439645 Mouse
- Unigene: 7102 Rat
References for Anti-TCP1 alpha antibody [23c ] (ab13501)
ab13501 has not yet been referenced specifically in any publications.
Publishing research using ab13501? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"