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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Overview
Product name
Anti-TCP1 alpha antibody [4E215]
See all TCP1 alpha products (11) ...
Description
Rat monoclonal [4E215] to TCP1 alpha
Specificity
This antibody recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. CTA-123 binds protein A and is useful for native precipitations of coatomer and CTT. This antibody does not react with human Hsp60 protein. This antibody also detects a molecular mass band of approximately 92kD on immunoblots.
Tested applications
WB, ICC, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog
Predicted to work with
Plants
Does not react with
Non Human Primates
Immunogen
Rats were immunized with a purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp-1b gene expressed in E. coli.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
4E215
Isotype
IgG2c
Research areas
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ICC
IP
Application notes
ICC: Use at a concentration of 5 µg/ml.
IP: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 [ECL] or 2 [Colorimetric] µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 67 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Sequence similarities
Belongs to the TCP-1 chaperonin family.
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome.
Target information above from: UniProt accessionP17987
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI528772 antibody
- c-cpn antibody
- CCT alpha antibody
see all
Database links
- Entrez Gene: 512043 Cow
- Entrez Gene: 484064 Dog
- Entrez Gene: 21454 Mouse
- Entrez Gene: 100302435 Rabbit
- Entrez Gene: 24818 Rat
- SwissProt: Q32L40 Cow
- SwissProt: P11983 Mouse
- SwissProt: P28480 Rat
see all
References for Anti-TCP1 alpha antibody [4E215] (ab14136)
ab14136 has not yet been referenced specifically in any publications.
Publishing research using ab14136? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"