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Product Name
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TCP1 alpha antibody [4E215]
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See all TCP1 alpha antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [4E215] to TCP1 alpha
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Immunogen
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Rats were immunized with a purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp-1b gene expressed in E. coli.
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Reacts with
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Ms, Rat, Cow, Dog, Hm, Plnts, Rb, Shp
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Does not react with
(species key)
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NHuPrm
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Specificity
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This antibody recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. CTA-123 binds protein A and is useful for native precipitations of coatomer and CTT. This antibody does not react with human Hsp60 protein. This antibody also detects a molecular mass band of approximately 92kD on immunoblots.
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Tested applications
(see key)
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ICC, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at a concentration of 5 µg/ml.
IP: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 [ECL] or 2 [Colorimetric] µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 67 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
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Relevance
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T complex polypeptide 1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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4E215
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Isotype
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IgG2c
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TCP1 alpha antibody [4E215] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to TCP1 alpha
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