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Product Name
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TCP1 alpha antibody [91A]
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See all TCP1 alpha antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [91A] to TCP1 alpha
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Immunogen
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Bacterially expressed fragment of mouse TCP-1, residues 306-556.
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Reacts with
(species key)
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Ms, Rat, Chk, Dog, Hm, NHuPrm, Sc
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Epitope
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The epitope for this antibody has been mapped to amino acids 465-469 representing the pentamer peptide AKLRA.
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Immunofluorescence: 1/500.
Western Blot: 1/1000.
Immunoprecipitation: Assay dependent.
Not tested in any other application.
Optimal dilutions / concentrations should be determined by the end user.
By Western blot, this clone detects a ~57 kDa band representing TCP 1 in murine 3T3 cells.
Immunofluorescence staining of TCP 1 in mouse germ line cells results in diffuse cytoplasmic staining.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
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Relevance
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T complex polypeptide 1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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91A
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Isotype
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IgG2a
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Purity
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Ascites
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Storage buffer
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PBS with 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TCP1 alpha antibody [91A] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for TCP1 alpha antibody [91A] (ab2797)
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This product has been used in:
Willison K et al. The t complex polypeptide 1 (TCP-1) is associated with the cytoplasmic aspect of Golgi membranes. Cell 57:621-32 (1989). PubMed: 2655925
If you publish research using ab2797 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to TCP1 alpha
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TCP1 alpha antibody [91A] - more information
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