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Western blot - TCP1 alpha antibody (ab10180)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCP1 alpha antibody (ab10180)

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Product Name
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TCP1 alpha antibody
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See all TCP1 alpha antibodies (7)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to TCP1 alpha
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Immunogen
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Synthetic peptide: SYEDAVHSGALND, corresponding to C terminal amino acids 544-556 of Human TCP1 alpha. BLAST 'C-SYEDAVHSGALND' with or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Chmp, Glla, Mcq (Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions Peptide ELISA: antibody detection limit dilution 1/32000. WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa according to NP_110379).
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human lung and testis lysates.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
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Relevance
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T complex polypeptide 1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: 0.5% BSA, Tris-saline. pH 7.3 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TCP1 alpha antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TCP1 alpha antibody images, references, products related to ab10180 and other tools.
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TCP1 alpha antibody images:
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Western blot - TCP1 alpha antibody (ab10180)
Predicted band size : 60 kDa
ab10180 staining (1µg/ml) of human lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCP1 alpha antibody (ab10180)
ab10180 (10µg/ml) staining paraffin embedded Human Testis, microwaved antigen retreival with Tris/EDTA buffer pH9, HRP-staining.
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Search PubMed (MEDLINE) for references to TCP1 alpha
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TCP1 alpha antibody - more information
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Customer reviews (feedback) regarding TCP1 alpha antibody |
Customer FAQs regarding TCP1 alpha antibody |
Protocols for TCP1 alpha antibody |
Price and availability of products related to TCP1 alpha antibody |
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TCP1 alpha antibody - related products:
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TCP1 alpha antibody - other tools:
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Contact Abcam with a Technical Enquiry about TCP1 alpha antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"