You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-TCP1 alpha antibody (ab10180)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-TCP1 alpha antibody
See all TCP1 alpha products (11) ...

Description

Goat polyclonal to TCP1 alpha

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Chimpanzee, Macaque Monkey, Gorilla

Immunogen

Synthetic peptide: SYEDAVHSGALND, corresponding to C terminal amino acids 544-556 of Human TCP1 alpha.

C-SYEDAVHS GALND

Positive control

Human lung and testis lysates.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - TCP1 alpha antibody (ab10180)Western blot - TCP1 alpha antibody (ab10180) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCP1 alpha antibody (ab10180)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCP1 alpha antibody (ab10180) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Peptide ELISA: antibody detection limit dilution 1/32000.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa according to NP_110379).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.

Sequence similarities

Belongs to the TCP-1 chaperonin family.

Cellular localization

Cytoplasm. Cytoplasm > cytoskeleton > centrosome.

Target information above from: UniProt accessionP17987 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AI528772 antibody
  • c-cpn antibody
  • CCT alpha antibody
  • CCT antibody
  • CCT-alpha antibody
  • CCT1 antibody
  • Ccta antibody
  • CCTalpha antibody
  • D6S230E antibody
  • MGC133746 antibody
  • p63 antibody
  • T complex 1 antibody
  • T complex locus TCP 1 antibody
  • T complex protein 1 alpha subunit antibody
  • T complex protein 1 antibody
  • T-complex homolog TCP1 antibody
  • T-complex protein 1 subunit alpha antibody
  • T-complex protein 1 subunit alpha B antibody
  • Tailless complex polypeptide 1 antibody
  • Tailless complex polypeptide 1A antibody
  • Tailless complex polypeptide 1B antibody
  • TCP 1 alpha antibody
  • Tcp-1 antibody
  • TCP-1-alpha antibody
  • TCP1 antibody
  • TCPA_HUMAN antibody
  • Tp63 antibody
  • TRic antibody
see all

Anti-TCP1 alpha antibody images:

  Western blot - TCP1 alpha antibody (ab10180)

Western blot - TCP1 alpha antibody (ab10180)



Predicted band size : 60 kDa


ab10180 staining (1µg/ml) of human lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCP1 alpha antibody (ab10180)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCP1 alpha antibody (ab10180)

ab10180 (10µg/ml) staining paraffin embedded Human Testis, microwaved antigen retreival with Tris/EDTA buffer pH9, HRP-staining.

References for Anti-TCP1 alpha antibody (ab10180)

This product has been referenced in:

  • Ryan Tet al. Identification of Novel Ryanodine Receptor 1 (RyR1) Protein Interaction with Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP). J Biol Chem 286:17060-8 (2011). WB; Human.Read more (PubMed: 21454501) »

See 1 publication for this product

Publishing research using ab10180? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"