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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Overview
Product name
Anti-TCP1 alpha antibody
See all TCP1 alpha products (11) ...
Description
Goat polyclonal to TCP1 alpha
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Chimpanzee, Macaque Monkey, Gorilla
Immunogen
Synthetic peptide: SYEDAVHSGALND, corresponding to C terminal amino acids 544-556 of Human TCP1 alpha.
C-SYEDAVHS GALND
Positive control
Human lung and testis lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Chaperones >> Other Chaperones
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
Peptide ELISA: antibody detection limit dilution 1/32000.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa according to NP_110379).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Sequence similarities
Belongs to the TCP-1 chaperonin family.
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome.
Target information above from: UniProt accessionP17987
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI528772 antibody
- c-cpn antibody
- CCT alpha antibody
see all
Database links
- Entrez Gene: 6950 Human
- Omim: 186980 Human
- SwissProt: P17987 Human
- Unigene: 363137 Human
Anti-TCP1 alpha antibody images:
Western blot - TCP1 alpha antibody (ab10180)
Predicted band size : 60 kDa
ab10180 staining (1µg/ml) of human lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCP1 alpha antibody (ab10180)
ab10180 (10µg/ml) staining paraffin embedded Human Testis, microwaved antigen retreival with Tris/EDTA buffer pH9, HRP-staining.
References for Anti-TCP1 alpha antibody (ab10180)
This product has been referenced in:
- Ryan Tet al. Identification of Novel Ryanodine Receptor 1 (RyR1) Protein Interaction with Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP). J Biol Chem 286:17060-8 (2011). WB; Human.Read more (PubMed: 21454501) »
See 1 publication for this product
Publishing research using ab10180? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"