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TCP1 beta protein (Tagged-His Tag) (ab91785)

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Overview

  • Product nameTCP1 beta protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 1-487 of Human TCP1 beta with N terminal His tag; MWt 56 kDa (UniProt P78371).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 68 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesMASLSLAPVNIFKAGADEERAETARLTSFIGAIAIGDLVK STLGPKGMDKILL SSGRDASLMVTNDGATILKNIGVDN PAAKVLVDMSRVQDDEVGDGTTSVTVLA AELLREAESL IAKKIHPQTIIAGWREATKAAREALLSSAVDHGSDEVKFR QDL MNIAGTTLSSKLLTHHKDHFTKLAVEAVLRLKGSG NLEAIHIIKKLGGSLADS YLDEGFLLDKKIGVNQPKRI ENAKILIANTGMDTDKIKIFGSRVRVDSTAKVA EIEHA EKEKMKEKVERILKHGINCFINRQLIYNYPEQLFGAAGVM AIEHADFA GVERLALVTGGEIASTFDHPELVKLGSCKL IEEVMIGEDKLIHFSGVALGEAC TIVLRGATQQILDEA ERSLHDALCVLAQTVKDSRTVYGGGCSEMLMAHAVTQL ANRTPGKEAVAMESYAKALRMLPTIIADNAGYDSADLVAQ LRAAHSEGNTTAG LDMREGTIGD
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91785 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        99D8.1CCT 2CCT beta
        CCT-betaCCT2CCTBChaperonin containing t complex polypeptide 1 beta subunitChaperonin containing t complex polypeptide 1 subunit 2Chaperonin containing TCP1 subunit 2Chaperonin containing TCP1 subunit 2 (beta)CTP:phosphocholine cytidylyltransferase 2MGC142074MGC142076MGC94480PRO1633T complex protein 1 beta subunitT complex protein 1 subunit betaT-complex protein 1 subunit betaTCP 1 betaTCP-1-betaTCPB_HUMAN
      see all
  • FunctionMolecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
  • Sequence similaritiesBelongs to the TCP-1 chaperonin family.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P78371 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    TCP1 beta protein (Tagged-His Tag) images

    • SDS-PAGE - TCP1 beta protein (Tagged-His Tag) (ab91785)
      SDS-PAGE - TCP1 beta protein (Tagged-His Tag) (ab91785)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for TCP1 beta protein (Tagged-His Tag) (ab91785)

    ab91785 has not yet been referenced specifically in any publications.

    Publishing research using ab91785 ? Please let us know so that we can cite the reference in this datasheet

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