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Western blot - TCTP antibody (ab37506)
Western blot - TCTP antibody (ab37506)
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Immunocytochemistry/ Immunofluorescence - TCTP antibody (ab37506)
Immunocytochemistry/ Immunofluorescence - TCTP antibody (ab37506)
(enlarge)


Western blot - TCTP antibody (ab37506)
Western blot - TCTP antibody (ab37506)
(enlarge)


Product Name 

TCTP antibody

 

See all TCTP antibodies (4)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to TCTP

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TCTP.

(Peptide available as ab37505.)

Reacts with 

Hu, Ms, Rat

Predicted to react 
(species key)

Chk, Cow, Pig, Rb
(Due to sequence homology)

Tested applications 
(see key)

ICC/IF, IHC-P, WB


Abreviews 

Customer reviews
(see key)


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• IHC-P

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IHC-P
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IHC-P
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Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: Use at a dilution of 1/2000.
WB: 1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive control in the follwing Human whole cell lysates:
HeLa
Jurkat
A431
HEK 293
HepG2
MCF-7
U2OS
This antibody gave a positive control in the follwing Mouse whole cell lysates:
NIH 3T3
MEF1
This antibody gave a positive control in the follwing Mouse tissue lysates:
Pancreas
Testis
Spinal Cord
Ovary
This antibody gave a positive control in the follwing Rat whole cell lysate:
PC12
This antibody gave a positive control in the follwing Rat tissue lysate:
Liver

Cellular localization 

Cytoplasmic

Research areas 

Immunology >> Immune System Diseases >> Allergens

Relevance 

TCTP is involved in calcium binding and microtubule stabilization. It is an immunoglobulin E dependent histamine-releasing factor produced by lymphocytes of atopic children and present in biologic fluids of allergic patients. Both human and mouse recombinant HRF proteins caused histamine release from human basophils of a subpopulation of donors (referenced from OMIM).

 

Alternative names TCTP antibody (ab37506)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

396363    (Chicken)
326599    (Cow)
7178    (Human)
22070    (Mouse)
397664    (Pig)
116646    (Rat)

Omim

    

600763    (Human)

SwissProt

    

P43347    (Chicken)
Q5E984    (Cow)
P13693    (Human)
Q5W0H4    (Human)
Q8TBK7    (Human)
Q3TMT0    (Mouse)
Q3UAG7    (Mouse)
Q3TWS8    (Mouse)
Q3TWX0    (Mouse)
Q569M9    (Mouse)
P63028    (Mouse)
P61288    (Pig)
P43348    (Rabbit)
P63029    (Rat)

Unigene

    

374596    (Human)
36610    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.20 - 0.50 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TCTP antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for TCTP antibody images, references, products related to ab37506 and other tools.

 

TCTP antibody images:

Product image  Western blot - TCTP antibody (ab37506)

Western blot - TCTP antibody (ab37506)

All lanes : TCTP antibody (ab37506) at 1/250 dilution

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

Performed under reducing conditions.

Predicted band size : 20 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)

 

Product image  Immunocytochemistry/ Immunofluorescence - TCTP antibody (ab37506)

Immunocytochemistry/ Immunofluorescence - TCTP antibody (ab37506)

ICC/IF image of ab37506 stained human HeLa cells. The cells were MeOH fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37506, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

 

Product image  Western blot - TCTP antibody (ab37506)

Western blot - TCTP antibody (ab37506)

All lanes : TCTP antibody (ab37506) at 1 µg/ml

Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Pancreas (Mouse) Tissue Lysate (ab29363)
Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 5 : Spinal Cord (Mouse) Tissue Lysate
Lane 6 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 8 : Liver (Rat) Tissue Lysate

Lysates/proteins at 10 µg per lane.

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 20 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.

 

References for TCTP antibody (ab37506)

Specific publication references   This product has been used in: (two most recent references)

Hartl D et al. Transcriptome and proteome analysis of early embryonic mouse brain development. Proteomics 8:1257-65 (2008). WB; Mouse. PubMed: 18283662

Kim JE et al. Identification of potential lung cancer biomarkers using an in vitro carcinogenesis model. Exp Mol Med 40:709-20 (2008). WB; Human. PubMed: 19116456
If you publish research using ab37506 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to TCTP

 

TCTP antibody - more information

Customer reviews (feedback) regarding TCTP antibody

Customer FAQs regarding TCTP antibody

Protocols for TCTP antibody

Price and availability of products related to TCTP antibody

 

TCTP antibody - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

Immunizing Peptide for TCTP antibody

ab37505

  

TCTP peptide

 

Compatible Secondaries for TCTP antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other TCTP Antibodies

See all TCTP antibodies (4)...

 

TCTP antibody - other tools:

Contact Abcam with a Technical Enquiry about TCTP antibody

 

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