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Western blot - TCTP antibody (ab37506)
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Immunocytochemistry/ Immunofluorescence - TCTP antibody (ab37506)
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Western blot - TCTP antibody (ab37506)
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Product Name
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TCTP antibody
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See all TCTP antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TCTP
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TCTP. (Peptide available as ab37505.)
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Chk, Cow, Pig, Rb
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: Use at a dilution of 1/2000.
WB: 1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive control in the follwing Human whole cell lysates:
HeLa
Jurkat
A431
HEK 293
HepG2
MCF-7
U2OS
This antibody gave a positive control in the follwing Mouse whole cell lysates:
NIH 3T3
MEF1
This antibody gave a positive control in the follwing Mouse tissue lysates:
Pancreas
Testis
Spinal Cord
Ovary
This antibody gave a positive control in the follwing Rat whole cell lysate:
PC12
This antibody gave a positive control in the follwing Rat tissue lysate:
Liver
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Cellular localization
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Cytoplasmic
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Research areas
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Immunology >> Immune System Diseases >> Allergens
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Relevance
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TCTP is involved in calcium binding and microtubule stabilization. It is an immunoglobulin E dependent histamine-releasing factor produced by lymphocytes of atopic children and present in biologic fluids of allergic patients. Both human and mouse recombinant HRF proteins caused histamine release from human basophils of a subpopulation of donors (referenced from OMIM).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.20 - 0.50 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TCTP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TCTP antibody images, references, products related to ab37506 and other tools.
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TCTP antibody images:
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 Immunocytochemistry/ Immunofluorescence - TCTP antibody (ab37506)
ICC/IF image of ab37506 stained human HeLa cells. The cells were MeOH fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37506, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Western blot - TCTP antibody (ab37506)
All lanes : TCTP antibody (ab37506) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Pancreas (Mouse) Tissue Lysate (ab29363)
Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 5 : Spinal Cord (Mouse) Tissue Lysate
Lane 6 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 8 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
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References for TCTP antibody (ab37506)
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This product has been used in: (two most recent references)
Hartl D et al. Transcriptome and proteome analysis of early embryonic mouse brain development. Proteomics 8:1257-65 (2008). WB; Mouse. PubMed: 18283662
Kim JE et al. Identification of potential lung cancer biomarkers using an in vitro carcinogenesis model. Exp Mol Med 40:709-20 (2008). WB; Human. PubMed: 19116456
If you publish research using ab37506 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to TCTP
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TCTP antibody - more information
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Customer reviews (feedback) regarding TCTP antibody |
Customer FAQs regarding TCTP antibody |
Protocols for TCTP antibody |
Price and availability of products related to TCTP antibody |
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TCTP antibody - related products:
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TCTP antibody - other tools:
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Contact Abcam with a Technical Enquiry about TCTP antibody |
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