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ab37505 |
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Read our guarantee »Products:Immunology >> Immune System Diseases >> Allergens
Anti-TCTP antibody
See all TCTP products (7) ...
Rabbit polyclonal to TCTP
IHC-Fr, WB, ICC/IF, IHC-Pmore details
Reacts with
Mouse, Rat, Human
Predicted to work with
Rabbit, Chicken, Cow, Pig
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TCTP.
(Peptide available as ab37505.)
This antibody gave a positive control in the follwing Human whole cell lysates: HeLa Jurkat A431 HEK 293 HepG2 MCF-7 U2OS This antibody gave a positive control in the follwing Mouse whole cell lysates: NIH 3T3 MEF1 This antibody gave a positive control in the follwing Mouse tissue lysates: Pancreas Testis Spinal Cord Ovary This antibody gave a positive control in the follwing Rat whole cell lysate: PC12 This antibody gave a positive control in the follwing Rat tissue lysate: Liver
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Our Abpromise guarantee covers the use of ab37506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent concentration. (PubMed: 22125622)
WB: 1/250Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
ICC/IF: Use a concentration of 5 µg/ml
IHC-P: 1/2000
Involved in calcium binding and microtubule stabilization.
Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate.
Belongs to the TCTP family.
Cytoplasm.
Target information above from: UniProt accessionP13693
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - TCTP antibody (ab37506)

All lanes : Anti-TCTP antibody (ab37506) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - TCTP antibody (ab37506)

ICC/IF image of ab37506 stained human HeLa cells. The cells were MeOH fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37506, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - TCTP antibody (ab37506)

All lanes : Anti-TCTP antibody (ab37506) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Pancreas (Mouse) Tissue Lysate (ab29363)
Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 5 : Spinal Cord (Mouse) Tissue Lysate
Lane 6 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 8 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
See all 8 publications for this product
Publishing research using ab37506? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:

All lanes : Anti-TCTP antibody (ab37506) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)

ICC/IF image of ab37506 stained human HeLa cells. The cells were MeOH fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37506, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

All lanes : Anti-TCTP antibody (ab37506) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Pancreas (Mouse) Tissue Lysate (ab29363)
Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 5 : Spinal Cord (Mouse) Tissue Lysate
Lane 6 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 8 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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