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Products:Immunology >> Immune System Diseases >> Allergens
Overview
Product name
Anti-TCTP antibody
See all TCTP products (7) ...
Description
Rabbit polyclonal to TCTP
Tested applications
IHC-Fr, WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Rabbit, Chicken, Cow, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TCTP.
(Peptide available as ab37505.)
Positive control
This antibody gave a positive control in the follwing Human whole cell lysates: HeLa Jurkat A431 HEK 293 HepG2 MCF-7 U2OS This antibody gave a positive control in the follwing Mouse whole cell lysates: NIH 3T3 MEF1 This antibody gave a positive control in the follwing Mouse tissue lysates: Pancreas Testis Spinal Cord Ovary This antibody gave a positive control in the follwing Rat whole cell lysate: PC12 This antibody gave a positive control in the follwing Rat tissue lysate: Liver
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent concentration. (PubMed: 22125622)
WB: 1/250Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
ICC/IF: Use a concentration of 5 µg/ml
IHC-P: 1/2000
Target
Function
Involved in calcium binding and microtubule stabilization.
Tissue specificity
Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate.
Sequence similarities
Belongs to the TCTP family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP13693
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ27337 antibody
- Fortilin antibody
- Histamine releasing factor antibody
see all
Database links
- Entrez Gene: 396363 Chicken
- Entrez Gene: 326599 Cow
- Entrez Gene: 7178 Human
- Entrez Gene: 22070 Mouse
- Entrez Gene: 397664 Pig
- Entrez Gene: 100008854 Rabbit
- Entrez Gene: 100344007 Rabbit
- Entrez Gene: 100353956 Rabbit
see all
Anti-TCTP antibody images:
Western blot - TCTP antibody (ab37506)
All lanes : Anti-TCTP antibody (ab37506) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - TCTP antibody (ab37506)
ICC/IF image of ab37506 stained human HeLa cells. The cells were MeOH fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37506, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - TCTP antibody (ab37506)
All lanes : Anti-TCTP antibody (ab37506) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Pancreas (Mouse) Tissue Lysate (ab29363)
Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 5 : Spinal Cord (Mouse) Tissue Lysate
Lane 6 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 8 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
References for Anti-TCTP antibody (ab37506)
This product has been referenced in:
- Skelton TSet al. Allochimeric molecules and mechanisms in abrogation of cardiac allograft rejection. J Heart Lung Transplant : (2011).Read more (PubMed: 21497519) »
- O'Dwyer Det al. The proteomics of colorectal cancer: identification of a protein signature associated with prognosis. PLoS One 6:e27718 (2011). IHC-Fr; Human.Read more (PubMed: 22125622) »
See all 8 publications for this product
Publishing research using ab37506? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"