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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCTP antibody (ab65015)
ab65015, at 1/50 - 1/100 dilution, staining TCTP in human breast carcinoma by Immunohistochemistry, using paraffin-embedded tissue, in the absence (left panel) or presence (right panel) of the immunising peptide. Antigen retrieval performed by boiling in a pressure cooker for 2-3 min. A polymer system was used for signal enhancing. Stained using DAB.
Western blot - TCTP antibody (ab65015)
All lanes : Anti-TCTP antibody (ab65015) at 1/500 dilution
Lane 1 : HepG2 cell extract (5-30 ug total protein)
Lane 2 : COLO205 cell extract (5-30 ug total protein)
Lane 3 : HeLa cell extract (5-30 ug total protein)
Lane 4 : HeLa cell extract with immunising peptide (5-10 ug)
Predicted band size : 20 kDa
Observed band size : 20 kDa
Immunocytochemistry/ Immunofluorescence-TCTP antibody(ab65015)
ICC/IF image of ab65015 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65015, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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