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Products:Immunology >> Immune System Diseases >> Allergens
Overview
Product name
Anti-TCTP antibody
See all TCTP products (7) ...
Description
Rabbit polyclonal to TCTP
Specificity
ab65015 detects endogenous levels of total TCTP protein.
Tested applications
ELISA, IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide derived from an internal sequence of human TCTP.
Positive control
Human breast carcinoma tissue. Extracts from HepG2, COLO205 and HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab65015 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
IHC-P: 1/50 - 1/100.Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB: 1/500 - 1/1000.Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ICC/IF: Use a concentration of 1 - 5 µg/ml.
Target
Function
Involved in calcium binding and microtubule stabilization.
Tissue specificity
Found in several healthy and tumoral cells including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas, and lymphomas. It cannot be detected in kidney and renal cell carcinoma (RCC). Expressed in placenta and prostate.
Sequence similarities
Belongs to the TCTP family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP13693
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ27337 antibody
- Fortilin antibody
- Histamine releasing factor antibody
see all
Database links
- Entrez Gene: 7178 Human
- Entrez Gene: 22070 Mouse
- Entrez Gene: 116646 Rat
- Omim: 600763 Human
- SwissProt: P13693 Human
- SwissProt: P63028 Mouse
- SwissProt: P63029 Rat
- Unigene: 374596 Human
see all
Anti-TCTP antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCTP antibody (ab65015)
ab65015, at 1/50 - 1/100 dilution, staining TCTP in human breast carcinoma by Immunohistochemistry, using paraffin-embedded tissue, in the absence (left panel) or presence (right panel) of the immunising peptide. Antigen retrieval performed by boiling in a pressure cooker for 2-3 min. A polymer system was used for signal enhancing. Stained using DAB.
Western blot - TCTP antibody (ab65015)
All lanes : Anti-TCTP antibody (ab65015) at 1/500 dilution
Lane 1 : HepG2 cell extract (5-30 ug total protein)
Lane 2 : COLO205 cell extract (5-30 ug total protein)
Lane 3 : HeLa cell extract (5-30 ug total protein)
Lane 4 : HeLa cell extract with immunising peptide (5-10 ug)
Predicted band size : 20 kDa
Observed band size : 20 kDa
Immunocytochemistry/ Immunofluorescence-TCTP antibody(ab65015)
ICC/IF image of ab65015 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65015, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-TCTP antibody (ab65015)
ab65015 has not yet been referenced specifically in any publications.
Publishing research using ab65015? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"