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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCTP antibody (ab65015)
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Western blot - TCTP antibody (ab65015)
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Immunocytochemistry/ Immunofluorescence-TCTP antibody(ab65015)
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Product Name
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TCTP antibody
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See all TCTP antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TCTP
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Immunogen
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Synthetic peptide derived from an internal sequence of human TCTP.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab65015 detects endogenous levels of total TCTP protein.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/20000.
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB: 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human breast carcinoma tissue. Extracts from HepG2, COLO205 and HeLa cells.
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Cellular localization
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Cytoplasmic
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Research areas
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Immunology >> Immune System Diseases >> Allergens
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Relevance
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TCTP is involved in calcium binding and microtubule stabilization. It is an immunoglobulin E dependent histamine-releasing factor produced by lymphocytes of atopic children and present in biologic fluids of allergic patients. Both human and mouse recombinant HRF proteins caused histamine release from human basophils of a subpopulation of donors (referenced from OMIM).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab65015 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TCTP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TCTP antibody images, references, products related to ab65015 and other tools.
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TCTP antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TCTP antibody (ab65015)
ab65015, at 1/50 - 1/100 dilution, staining TCTP in human breast carcinoma by Immunohistochemistry, using paraffin-embedded tissue, in the absence (left panel) or presence (right panel) of the immunising peptide. Antigen retrieval performed by boiling in a pressure cooker for 2-3 min. A polymer system was used for signal enhancing. Stained using DAB.
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Western blot - TCTP antibody (ab65015)
All lanes : TCTP antibody (ab65015) at 1/500 dilution
Lane 1 : HepG2 cell extract (5-30 ug total protein)
Lane 2 : COLO205 cell extract (5-30 ug total protein)
Lane 3 : HeLa cell extract (5-30 ug total protein)
Lane 4 : HeLa cell extract with immunising peptide (5-10 ug)
Predicted band size : 20 kDa
Observed band size : 20 kDa
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Immunocytochemistry/ Immunofluorescence-TCTP antibody(ab65015)
ICC/IF image of ab65015 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65015, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to TCTP
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TCTP antibody - more information
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Customer reviews (feedback) regarding TCTP antibody |
Customer FAQs regarding TCTP antibody |
Protocols for TCTP antibody |
Price and availability of products related to TCTP antibody |
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TCTP antibody - related products:
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TCTP antibody - other tools:
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Contact Abcam with a Technical Enquiry about TCTP antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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