The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at a concentration of 2-5 µg/mg lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
Ubiquitous in fetal and adult tissues.
Involvement in disease
Note=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein. Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.
Belongs to the MiT/TFE family. Contains 1 basic helix-loop-helix (bHLH) domain.
Sumoylated; does not affect dimerization with MITF.
Class E basic helix-loop-helix protein 33 antibody
RCCP 2 antibody
Renal cell carcinoma, papillary antibody
Tcfe 3 antibody
TFE 3 antibody
Transcription factor binding to IGHM enhancer 3 antibody
Transcription factor E family, member A antibody
Transcription factor E3 antibody
Transcription factor for IgH enhancer antibody
Transcription factor for immunoglobulin heavy chain enhancer 3 antibody
Western blot - TFE3 antibody (ab93808)
All lanes : Anti-TFE3 antibody (ab93808) at 0.04 µg/ml
Lane 1 : HeLa whole lysate at 50 µg Lane 2 : HeLa whole lysate at 15 µg Lane 3 : HeLa whole lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 62 kDa
Exposure time: 3 minutes
Immunoprecipitation - TFE3 antibody (ab93808)
1 mg HeLa cell lysate was immunoprecipitated with 3µg/mg ab93808 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitates were analysed by Western blot. Bands were analysed using ab93808 at 0.4µg/ml and developed using cheiluminescence.