• Product nameAnti-TFE3 antibody
    See all TFE3 primary antibodies
  • Description
    Rabbit polyclonal to TFE3
  • Tested applicationsWB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Elephant
  • Immunogen

    Synthetic peptide from a region within amino acids 500-550 of Human TFE3 (NP_006512.2)

  • Positive control
    • HeLa and 293T cells



Our Abpromise guarantee covers the use of ab93808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIP: Use at a concentration of 2-5 µg/mg lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 62 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
    • Tissue specificityUbiquitous in fetal and adult tissues.
    • Involvement in diseaseNote=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
      Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.
    • Sequence similaritiesBelongs to the MiT/TFE family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      Sumoylated; does not affect dimerization with MITF.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLH e33 antibody
      • bHLHe33 antibody
      • Class E basic helix-loop-helix protein 33 antibody
      • RCCP 2 antibody
      • RCCP2 antibody
      • RCCX1 antibody
      • Renal cell carcinoma, papillary antibody
      • Tcfe 3 antibody
      • Tcfe3 antibody
      • TFE 3 antibody
      • Tfe3 antibody
      • TFE3_HUMAN antibody
      • TFEA antibody
      • Transcription factor binding to IGHM enhancer 3 antibody
      • Transcription factor E family, member A antibody
      • Transcription factor E3 antibody
      • Transcription factor for IgH enhancer antibody
      • Transcription factor for immunoglobulin heavy chain enhancer 3 antibody
      see all

    Anti-TFE3 antibody images

    • All lanes : Anti-TFE3 antibody (ab93808) at 0.04 µg/ml

      Lane 1 : HeLa whole lysate at 50 µg
      Lane 2 : HeLa whole lysate at 15 µg
      Lane 3 : HeLa whole lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg

      developed using the ECL technique

      Predicted band size : 62 kDa

      Exposure time : 3 minutes
    • 1 mg HeLa cell lysate was immunoprecipitated with 3µg/mg ab93808 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitates were analysed by Western blot. Bands were analysed using ab93808 at 0.4µg/ml and developed using cheiluminescence.

    References for Anti-TFE3 antibody (ab93808)

    ab93808 has not yet been referenced specifically in any publications.

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