Overview

  • Product nameAnti-TFE3 antibodySee all TFE3 primary antibodies ...
  • Description
    Rabbit polyclonal to TFE3
  • Tested applicationsWB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Elephant
  • Immunogen

    Synthetic peptide from a region within amino acids 500-550 of Human TFE3 (NP_006512.2)

  • Positive control
    • HeLa and 293T cells

Properties

Applications

Our Abpromise guarantee covers the use of ab93808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at a concentration of 2-5 µg/mg lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 62 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
    • Tissue specificityUbiquitous in fetal and adult tissues.
    • Involvement in diseaseNote=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
      Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.
    • Sequence similaritiesBelongs to the MiT/TFE family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      modifications
      Sumoylated; does not affect dimerization with MITF.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLH e33 antibody
      • bHLHe33 antibody
      • Class E basic helix-loop-helix protein 33 antibody
      • RCCP 2 antibody
      • RCCP2 antibody
      • RCCX1 antibody
      • Renal cell carcinoma, papillary antibody
      • Tcfe 3 antibody
      • Tcfe3 antibody
      • TFE 3 antibody
      • Tfe3 antibody
      • TFE3_HUMAN antibody
      • TFEA antibody
      • Transcription factor binding to IGHM enhancer 3 antibody
      • Transcription factor E family, member A antibody
      • Transcription factor E3 antibody
      • Transcription factor for IgH enhancer antibody
      • Transcription factor for immunoglobulin heavy chain enhancer 3 antibody
      see all

    Anti-TFE3 antibody images

    • All lanes : Anti-TFE3 antibody (ab93808) at 0.04 µg/ml

      Lane 1 : HeLa whole lysate at 50 µg
      Lane 2 : HeLa whole lysate at 15 µg
      Lane 3 : HeLa whole lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg

      developed using the ECL technique

      Predicted band size : 62 kDa


      Exposure time : 3 minutes
    • 1 mg HeLa cell lysate was immunoprecipitated with 3µg/mg ab93808 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitates were analysed by Western blot. Bands were analysed using ab93808 at 0.4µg/ml and developed using cheiluminescence.

    References for Anti-TFE3 antibody (ab93808)

    ab93808 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab93808.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"