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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIIB
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
Overview
Product name
Anti-TFIIB (acetyl K238) antibody
See all TFIIB products (13) ...
Description
Rabbit polyclonal to TFIIB (acetyl K238)
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Cross reactivity
Predicted to work with
Mouse, Human, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human TFIIB, acetylated at K238.
(Peptide available as ab26407.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Application notes
This antibody gave a positive result in ELISA against the immunizing peptide (ab26407). It gave a negative result in ELISA against the non-modified equivalent peptide (ab25428). This indicates that it is specific for the modified peptide.
See figure below.
IF/ICC: See figure below.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Sequence similarities
Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ00403
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- General transcription factor IIB antibody
- General transcription factor TFIIB antibody
- GTF2B antibody
see all
Database links
- Entrez Gene: 2959 Human
- Entrez Gene: 229906 Mouse
- Entrez Gene: 394390 Xenopus laevis
- Omim: 189963 Human
- SwissProt: Q00403 Human
- SwissProt: P62915 Mouse
- SwissProt: P29054 Xenopus laevis
- Unigene: 481852 Human
see all
Anti-TFIIB (acetyl K238) antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
ELISA - TFIIB (acetyl K238) antibody (ab5210)
This antibody gave a positive result in ELISA against the immunising peptide (ab26407) and a negative result using the non-modified equivalent peptide (ab25428). This indicates that it is specific for the modified peptide.
Red line: ELISA against immunising peptide ab26407
Green line: ELISA against non-modified peptide ab25428
Immunocytochemistry/ Immunofluorescence - TFIIB (acetyl K238) antibody (ab5210)
ICC/IF image of ab5210 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab5210, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and Mcf7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
References for Anti-TFIIB (acetyl K238) antibody (ab5210)
ab5210 has not yet been referenced specifically in any publications.
Publishing research using ab5210? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"