Recombinant Anti-TFIIB antibody [EPR4589] (ab109106)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4589] to TFIIB
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TFIIB antibody [EPR4589]
See all TFIIB primary antibodies -
Description
Rabbit monoclonal [EPR4589] to TFIIB -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, HeLa, Jurkat, K562, C6, RAW 264.7, and PC12 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4589 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
Target
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Function
General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. -
Sequence similarities
Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2959 Human
- Entrez Gene: 229906 Mouse
- Entrez Gene: 81673 Rat
- Omim: 189963 Human
- SwissProt: Q00403 Human
- SwissProt: P62915 Mouse
- SwissProt: P62916 Rat
- Unigene: 481852 Human
see all -
Alternative names
- General transcription factor IIB antibody
- General transcription factor TFIIB antibody
- gtf2b antibody
see all
Images
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All lanes : Anti-TFIIB antibody [EPR4589] (ab109106) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW 264.7 cell lysate
Lane 7 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab109106 has been referenced in 3 publications.
- Sun YT et al. Overexpression of lncRNA HCP5 in human umbilical cord mesenchymal stem cell-derived exosomes promoted the proliferation and inhibited the apoptosis of ovarian granulosa cells via the musashi RNA-binding protein 2/oestrogen receptor alpha 1 axis. Endocr J 69:1117-1129 (2022). PubMed: 35545536
- Ruan Z et al. Overexpression of long non-coding RNA00355 enhances proliferation, chemotaxis, and metastasis in colon cancer via promoting GTF2B-mediated ITGA2. Transl Oncol 14:100947 (2021). PubMed: 33227664
- Yang J et al. Targeting LOXL2 for cardiac interstitial fibrosis and heart failure treatment. Nat Commun 7:13710 (2016). WB . PubMed: 27966531