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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIIB
Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094)
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DNA RNA and cDNA
ab46666
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab12094? Please let us know so that we can cite the reference in this datasheet
ab12094 has been referenced in 1 publications.
- Deng W et al. TFIIB recognition elements control the TFIIA-NC2 axis in transcriptional regulation. Mol Cell Biol 29:1389-400 (2009). WB, ChIP; Human. PubMed: 19114554
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![ChIP - TFIIB antibody [IIB1] (ab12094)](/ps/datasheet/Images/12/ab12094/ab12094_1.jpg)
ChIP - TFIIB antibody [IIB1] (ab12094)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 8µl of ab12094 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).
![Western blot - Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094)](/ps/datasheet/images/12/ab12094/TFIIB-Primary-antibodies-ab12094-2.jpg)
Western blot - Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094)
Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094) at 1/1000 dilution +
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
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