Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIIB
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ab46666 |
If your product does not perform as described on this datasheet, we will refund or replace your product...
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Thanks a lot for your reply. I decided to buy the antibody and test it in Drosophila. Please send me the discount code. Thank you very much |
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ANSWER: |
I am very pleased to hear you would like to accept our offer and test ab12094 in drosophila. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview fordrosophila and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. |
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I am interested in the Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094). Since TFIIB is very conserved, I wonder if the antibody will also work in Drosophila (fruit fly)? Have your company tested it? or do you know it has been used in another Drosophila lab before? If you have no information about this particular antibody for Drosophila works, do you know any of your TFIIB antibody would react with the Drosophila TFIIB? Looking forward to your reply. Thanks a lot Have a nice day, |
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ANSWER: |
Thank you very much for your interest in ab12094. To our knowledge, ab12094 has not been tested in drosophila. Therefore, I can offer a discount off a future purchase if you buy ab12094 now, test it in drosophila and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab12094 in drosophila. I will then send a discount code. This code must be issued before purchasing ab12094 so please wait for my reply before ordering. 2. Purchase ab12094 either by phone, fax, or online (www.abcam.com). 3. Test it in drosophila. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab12094 turns out to be unsuitable for drosophila, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 8µl of ab12094 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).
Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094) at 1/1000 dilution +
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
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