![ChIP - TFIIB antibody [IIB1] (ab12094) image](#ab12094_1.jpg)
![Western blot - Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094) image](#TFIIB-Primary-antibodies-ab12094-2.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIIB
Overview
Product name
Anti-TFIIB antibody [IIB1] - ChIP Grade
See all TFIIB products (13) ...
Description
Mouse monoclonal [IIB1] to TFIIB - ChIP Grade
Tested applications
ChIP, WB, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
all Mammals
Immunogen
Human TFIIB eukaryotic transcription factor.
Epitope
Ab12094 recognises epitope 1-51.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Raw Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
IIB1
Isotype
IgG2b
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12094 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use 5-10 µg for 25 µg of chromatin.
WB: 1/1000Predicted molecular weight: 35 kDa.
IP: Use at an assay dependent dilution.
Target
Function
General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Sequence similarities
Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ00403
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- General transcription factor IIB antibody
- General transcription factor TFIIB antibody
- gtf2b antibody
see all
Database links
- Entrez Gene: 2959 Human
- Omim: 189963 Human
- SwissProt: Q00403 Human
- Unigene: 481852 Human
Anti-TFIIB antibody [IIB1] - ChIP Grade images:
ChIP - TFIIB antibody [IIB1] (ab12094)
![ChIP - TFIIB antibody [IIB1] (ab12094)](/ps/datasheet/Images/12/ab12094/ab12094_1.jpg)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 8µl of ab12094 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).
Western blot - Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094)
![Western blot - Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094)](/ps/datasheet/images/12/ab12094/TFIIB-Primary-antibodies-ab12094-2.jpg)
Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094) at 1/1000 dilution + TFIIB protein (His tag) (ab81898) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
References for Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094)
This product has been referenced in:
- Deng Wet al. TFIIB recognition elements control the TFIIA-NC2 axis in transcriptional regulation. Mol Cell Biol 29:1389-400 (2009). WB, ChIP; Human.Read more (PubMed: 19114554) »
See 1 publication for this product
Publishing research using ab12094? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"