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Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094)

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Overview

Product name

Anti-TFIIB antibody [IIB1] - ChIP Grade
See all TFIIB products (13) ...

Description

Mouse monoclonal [IIB1] to TFIIB - ChIP Grade

Tested applications

ChIP, WB, IPmore details

Cross reactivity

Reacts with

Human

Predicted to work with

all Mammals

Immunogen

Human TFIIB eukaryotic transcription factor.

Epitope

Ab12094 recognises epitope 1-51.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Raw Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

IIB1

Isotype

IgG2b

  • ChIP - TFIIB antibody [IIB1] (ab12094)ChIP - TFIIB antibody [IIB1] (ab12094) image (enlarge)

  • Western blot - Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094)Western blot - Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab12094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.

Sequence similarities

Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ00403 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • General transcription factor IIB antibody
  • General transcription factor TFIIB antibody
  • gtf2b antibody
  • RNA polymerase II transcription factor IIB antibody
  • S300 II antibody
  • S300-II antibody
  • TF IIB antibody
  • TF2B antibody
  • TF2B_HUMAN antibody
  • Transcription initiation factor IIB antibody
see all

Anti-TFIIB antibody [IIB1] - ChIP Grade images:

  ChIP - TFIIB antibody [IIB1] (ab12094)

ChIP - TFIIB antibody [IIB1] (ab12094)

Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 8µl of  ab12094 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).

  Western blot - Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094)

Western blot - Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094)

Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094) at 1/1000 dilution + TFIIB protein (His tag) (ab81898) at 0.01 µg

Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Exposure time : 10 seconds

References for Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094)

This product has been referenced in:

  • Deng Wet al. TFIIB recognition elements control the TFIIA-NC2 axis in transcriptional regulation. Mol Cell Biol 29:1389-400 (2009). WB, ChIP; Human.Read more (PubMed: 19114554) »

See 1 publication for this product

Publishing research using ab12094? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"