You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-TFIIB antibody [IIB8] (ab819)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-TFIIB antibody [IIB8]
See all TFIIB products (13) ...

Description

Mouse monoclonal [IIB8] to TFIIB

Tested applications

Functional Studies, EMSA, ELISA, IP, Purification, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Partially purified recombinant human transcription factor IIB (TFIIB, Promega Corp.).

Epitope

Amino acids 52-105 of human and mouse TFIIB.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS with 0.02% sodium azide

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Monoclonal

Clone number

IIB8

Isotype

IgG2a

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Preinitiation complex
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIIB

Applications

Show applications key

Our Abpromise guarantee covers the use of ab819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    Functional Studies

     FuncS: Use at an assay d...Read more →

    FuncS: Use at an assay dependent dilution. (This antibody can also immuno-deplete TFIIB from cell nuclear extracts and inhibit transcription of TFIIB from cell nuclear extracts.)

  • ShowHide

    EMSA

     EMSA: Use at an assay de...Read more →

    EMSA: Use at an assay dependent dilution.

  • ShowHide

    ELISA

     ELISA: Use at an assay d...Read more →

    ELISA: Use at an assay dependent dilution.

  • ShowHide

    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

  • ShowHide

    Purification

     P: Use at an assay depen...Read more →

    P: Use at an assay dependent dilution. (This antibody is polyol-responsive and can be used for gentle purification of TFIIB.)

  • ShowHide

    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent dilution. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).

Target

Function

General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.

Sequence similarities

Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ00403 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • General transcription factor IIB antibody
  • General transcription factor TFIIB antibody
  • gtf2b antibody
  • RNA polymerase II transcription factor IIB antibody
  • S300 II antibody
  • S300-II antibody
  • TF IIB antibody
  • TF2B antibody
  • TF2B_HUMAN antibody
  • Transcription initiation factor IIB antibody
see all

Database links

References for Anti-TFIIB antibody [IIB8] (ab819)

This product has been referenced in:

  • Paes de Faria J & Fernandes L Protection against oxidative stress through SUA7/TFIIB regulation in Saccharomyces cerevisiae. Free Radic Biol Med 41:1684-93 (2006). WB; Saccharomyces cerevisiae.Read more (PubMed: 17145557) »
  • Thompson NEet al. Accessibility of epitopes on human transcription factor IIB in the native protein and in a complex with DNA. J Biol Chem 270:4735-40 (1995).Read more (PubMed: 7533165) »

See all 3 publications for this product

Publishing research using ab819? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"