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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIIB
Overview
Product name
Anti-TFIIB antibody
See all TFIIB products (13) ...
Description
Rabbit polyclonal to TFIIB
Specificity
This antibody recognizes TFIIB
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Zebrafish
Immunogen
A region within synthetic peptide: SSDRAMMNAF KEITTMADRI NLPRNIVDRT NNLFKQVYEQ KSLKGRANDA, corresponding to N terminal amino acids 109-158 of Human TFIIB
SSDRAMMNAF KEITTMADRI NLPRNIVDRT NNLFKQVYEQ KSLKGRANDA
Positive control
Hep G2 cell lysate, human intenstine
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: Use at an assay dependent dilution.
Note: ELISA titre using peptide based assay: 1:312500
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 4 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Sequence similarities
Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ00403
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- General transcription factor IIB antibody
- General transcription factor TFIIB antibody
- GTF2B antibody
see all
Database links
- Entrez Gene: 2959 Human
- Entrez Gene: 229906 Mouse
- Entrez Gene: 81673 Rat
- Omim: 189963 Human
- SwissProt: Q00403 Human
- SwissProt: P62915 Mouse
- SwissProt: P62916 Rat
- Unigene: 481852 Human
see all
Anti-TFIIB antibody images:
Western blot - TFIIB antibody (ab49135)
Anti-TFIIB antibody (ab49135) at 4 µg/ml + HepG2 cell lysate at 10 µg with 5% skimmed milk in PBS
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 35 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
Immunohistochemistry (Paraffin-embedded sections) - TFIIB antibody (ab49135)
ab49135 at 4µg/ml staining Human intestine using paraffin-embeded sections.
References for Anti-TFIIB antibody (ab49135)
ab49135 has not yet been referenced specifically in any publications.
Publishing research using ab49135? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"