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TFIIB protein (His tag) (ab81898)

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Overview

Product name

TFIIB protein (His tag)
See all TFIIB products (5) ...

Protein description

Recombinant full length human TFIIB with His tag; amino acids 1-316, Predicted MWt 35kDa (SwissProt Q00403).

Expression host

E. coli

Properties

Purity

>95% by SDS-PAGE

Form

Liquid

Storage instructions

Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

Concentration

Concentration information loading...

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Preinitiation complex
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIIB

  • Western blot - Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094)Western blot - Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094) image (enlarge)

Applications

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Our Abpromise guarantee covers the use of ab81898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    Gel supershift assays

     GSA: Use at an assay dep...Read more →

    GSA: Use at an assay dependent dilution. (Use 1 unit in a 20 µl reaction. )

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    SDS-PAGE

     SDS-PAGE: Use at an ass...Read more →

    SDS-PAGE: Use at an assay dependent dilution.

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    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent concentration. (Ab81898 can be used as a WB positive control in conjunction with ab12094. )

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    Other

     Other: Use at an assay d...Read more →

    Other: Use at an assay dependent concentration. (Ab81898 has been used for in vitro transcription assays and protein-protein interaction assays. 10 units are sufficient for a 25 µl reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay detected by immuno-blot system. 1 unit equals 1 nanogram of purified protein. )

Protein info

Function

General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.

Sequence similarities

Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ00403 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

TFIIB protein (His tag) images:

  Western blot - Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094)

Western blot - Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094)

Anti-TFIIB antibody [IIB1] - ChIP Grade (ab12094) at 1/1000 dilution + TFIIB protein (His tag) (ab81898) at 0.01 µg

Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Exposure time : 10 seconds

References for TFIIB protein (His tag) (ab81898)

ab81898 has not yet been referenced specifically in any publications.

Publishing research using ab81898? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"