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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIIB
Overview
Product name
TFIIB protein (Tagged)
See all TFIIB products (5) ...
Protein description
Recombinant full length human TFIIB with proprietary tag, Estimated MWt 61kDa (Swiss-Prot Q00403).
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Form
Liquid
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
Concentration
Concentration information loading...
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE
Application notes
SDS-PAGE: Use at an assay dependent dilution.
Protein-protein interaction assay: 100 units are sufficient when detected by an immuno-blot system.
1 unit equals 1 nanogram of purified protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Sequence similarities
Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ00403
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for TFIIB protein (Tagged) (ab81930)
ab81930 has not yet been referenced specifically in any publications.
Publishing research using ab81930? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"