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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription
Overview
Product name
Anti-TFIIF antibody [2A15]
See all TFIIF products (5) ...
Description
Mouse monoclonal [2A15] to TFIIF
Specificity
Recognizes human Transcription Factor IIF (RAP30).
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length native protein (purified) (Human).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
0.1M Tris HCl, pH 7.5
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
2A15
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 28.3 kDa.
ELISA: Use at an assay dependent dilution.
Target
Relevance
The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
Cellular localization
Nuclear
Alternative names
- ATP dependent helicase GTF2F2 antibody
- BTF 4 antibody
- BTF4 antibody
see all
Database links
- Entrez Gene: 2962 Human
- Entrez Gene: 2963 Human
- Omim: 189968 Human
- Omim: 189969 Human
- SwissProt: P13984 Human
- SwissProt: P35269 Human
- Unigene: 654582 Human
- Unigene: 68257 Human
References for Anti-TFIIF antibody [2A15] (ab4449)
This product has been referenced in:
- Orphanides Get al. The general transcription factors of RNA polymerase II. Genes Dev 10:2657-83 (1996).Read more (PubMed: 8946909) »
- Wang BQet al. Importance of codon preference for production of human RAP74 and reconstitution of the RAP30/74 complex. Protein Expr Purif 5:476-85 (1994).Read more (PubMed: 7827505) »
See all 3 publications for this product
Publishing research using ab4449? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"