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ChIP - TFIIF antibody - ChIP Grade (ab28179)
ChIP - TFIIF antibody - ChIP Grade (ab28179)
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Product Name 

TFIIF antibody - ChIP Grade

 

See all TFIIF antibodies (5)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to TFIIF - ChIP Grade

Immunogen 

Recombinant full length protein - RAP 74 subunit of TF11F.

Reacts with 
(species key)

Hu

Specificity 

This antibody reacts with the RAP74 subunit of TFIIF.

Tested applications 
(see key)

ChIP, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ChIP: Use 5-10µg for 25µg of chromatin
WB: 1/2000 - 1/5000. Predicted molecular weight: 58 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cell lysate.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIIF

Relevance 

The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD.
TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template.
TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.

 

Alternative names TFIIF antibody - ChIP Grade (ab28179)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

2963    (Human)
2962    (Human)

Omim

    

189969    (Human)
189968    (Human)

SwissProt

    

P13984    (Human)
P35269    (Human)

Unigene

    

68257    (Human)
654582    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TFIIF antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance!


See below for TFIIF antibody - ChIP Grade images, references, products related to ab28179 and other tools.

 

TFIIF antibody - ChIP Grade images:

Product image  ChIP - TFIIF antibody - ChIP Grade (ab28179)

ChIP - TFIIF antibody - ChIP Grade (ab28179)

    Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The  ChIP was performed with 25µg of chromatin, 8µg of ab28179 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.

 
 

Search PubMed (MEDLINE) for references to TFIIF

 

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