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ChIP - TFIIF antibody - ChIP Grade (ab28179)
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Product Name
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TFIIF antibody - ChIP Grade
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See all TFIIF antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TFIIF - ChIP Grade
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Immunogen
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Recombinant full length protein - RAP 74 subunit of TF11F.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody reacts with the RAP74 subunit of TFIIF.
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Tested applications
(see key)
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ChIP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use 5-10µg for 25µg of chromatin
WB: 1/2000 - 1/5000. Predicted molecular weight: 58 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIIF
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Relevance
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The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD.
TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template.
TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TFIIF antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TFIIF antibody - ChIP Grade images, references, products related to ab28179 and other tools.
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TFIIF antibody - ChIP Grade images:
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 ChIP - TFIIF antibody - ChIP Grade (ab28179)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 25µg of chromatin, 8µg of ab28179 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.
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Search PubMed (MEDLINE) for references to TFIIF
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TFIIF antibody - ChIP Grade - more information
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Customer reviews (feedback) regarding TFIIF antibody - ChIP Grade |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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