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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription
Overview
Product name
Anti-TFIIF antibody - ChIP Grade
See all TFIIF products (5) ...
Description
Rabbit polyclonal to TFIIF - ChIP Grade
Specificity
This antibody reacts with the RAP74 subunit of TFIIF.
Tested applications
WB, ChIPmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein - RAP 74 subunit of TF11F.
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIIF
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription
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ChIP - TFIIF antibody - ChIP Grade (ab28179)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab28179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/5000.Predicted molecular weight: 58 kDa.
ChIP: Use 5-10 µg for 25 µg of chromatin.
Target
Relevance
The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
Cellular localization
Nuclear
Alternative names
- ATP dependent helicase GTF2F2 antibody
- TFIIF alpha antibody
- TFIIF beta antibody
see all
Database links
- Entrez Gene: 2962 Human
- Entrez Gene: 2963 Human
- Omim: 189968 Human
- Omim: 189969 Human
- SwissProt: P13984 Human
- SwissProt: P35269 Human
- Unigene: 654582 Human
- Unigene: 68257 Human
Anti-TFIIF antibody - ChIP Grade images:
ChIP - TFIIF antibody - ChIP Grade (ab28179)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 25µg of chromatin, 8µg of ab28179 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.
References for Anti-TFIIF antibody - ChIP Grade (ab28179)
ab28179 has not yet been referenced specifically in any publications.
Publishing research using ab28179? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"