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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription
Overview
Product name
Anti-TFIIF antibody
See all TFIIF products (5) ...
Description
Rabbit polyclonal to TFIIF
Specificity
This antibody reacts with Transcription initiation factor IIF beta subunit (GTF2F2), polypeptide 2, 30kDa.
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog
Immunogen
Synthetic peptide: QLDKVVTTNY KPVANHQYNI EYERKKKEDG KRARADKQHV LDMLFSAFEK, corresponding to internal sequence amino acids 144-193 of Human TFIIF
QLDKVVTTNY KPVANHQYNI EYERKKKEDG KRARADKQHV LDMLFSAFEK
Positive control
HepG2 cell lysate, Human brain tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1,562,500ELISA: 1/1,562,500
WB: Use a concentration of 1 µg/mlDetects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
Target
Relevance
The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
Cellular localization
Nuclear
Alternative names
- ATP dependent helicase GTF2F2 antibody
- TFIIF alpha antibody
- TFIIF beta antibody
see all
Database links
- Entrez Gene: 505702 Cow
- Entrez Gene: 2962 Human
- Entrez Gene: 2963 Human
- Entrez Gene: 68705 Mouse
- Entrez Gene: 98053 Mouse
- Entrez Gene: 316123 Rat
- Entrez Gene: 81674 Rat
- Omim: 189968 Human
see all
Anti-TFIIF antibody images:
Western blot - TFIIF antibody (ab49154)
Anti-TFIIF antibody (ab49154) at 1 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG 1/50,000 - 1/100,000
Predicted band size : 28 kDa
Observed band size : 28 kDa
References for Anti-TFIIF antibody (ab49154)
ab49154 has not yet been referenced specifically in any publications.
Publishing research using ab49154? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"