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Western blot - TFIIF antibody (ab49154)
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Immunohistochemistry (Paraffin-embedded sections) - TFIIF antibody (ab49154)
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Product Name
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TFIIF antibody
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See all TFIIF antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TFIIF
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Immunogen
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Synthetic peptide: QLDKVVTTNY KPVANHQYNI EYERKKKEDG KRARADKQHV LDMLFSAFEK, corresponding to internal sequence amino acids 144-193 of Human TFIIF
BLAST 'QLDKVVTTNY KPVANHQYNI EYERKKKEDG KRARADKQHV LDMLFSAFEK ' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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This antibody reacts with Transcription initiation factor IIF beta subunit (GTF2F2), polypeptide 2, 30kDa.
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1,562,500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cell lysate, Human brain tissue.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIIF
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Relevance
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The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD.
TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template.
TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TFIIF antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TFIIF antibody images, references, products related to ab49154 and other tools.
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TFIIF antibody images:
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 Western blot - TFIIF antibody (ab49154)
TFIIF antibody (ab49154) at 1 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG 1/50,000 - 1/100,000
Predicted band size : 28 kDa
Observed band size : 28 kDa
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Immunohistochemistry (Paraffin-embedded sections) - TFIIF antibody (ab49154)
This image shows human brain tissue stained with ab49154 at 8µg/ml. Arrows pointing to neural cells indicate positive labelling. Magnification X400.
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Search PubMed (MEDLINE) for references to TFIIF
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TFIIF antibody - more information
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Customer reviews (feedback) regarding TFIIF antibody |
Customer FAQs regarding TFIIF antibody |
Protocols for TFIIF antibody |
Price and availability of products related to TFIIF antibody |
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TFIIF antibody - related products:
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TFIIF antibody - other tools:
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Contact Abcam with a Technical Enquiry about TFIIF antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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