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- Proteins and Peptides
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab9881 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
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I have just purchased the above-named antibody from you and would like to test it on human endothelial cells (Western Blot). I have the following questions: 1) Can I use reducing conditions for my samples (i.e., use DTT, BME), or does it have to be non-reducing? 2) Do you block / dissolve the antibody in 5% milk powder or in BSA (bovine serum albumine)? Thank you very much for your quick help!
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ANSWER: |
Thank you for your enquiry. You can use reducing Western blotting conditions (if non-reducing conditions are required, we would state this information on the online datasheet). We would recommend blocking with either 5% w/v nonfat dry milk or 5% w/v BSA in PBST or TBST. If you have any additional questions, please contact us again. |
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I WANT TO USE THE TFPI Ab FOR FACS ANALYSIS. IS IT SUITABLE?...I KNOW THAT THE ONLY APPLICATION LISTED IS WESTERN BLOTTING BUT IF YOU HAVE MORE INFO PLEASE LET ME KNOW URGENTLY SO I CAN ORDER IT STRAIGHT AWAY.
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ANSWER: |
Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in FACS. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. |
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does the antibody cross-react with mouse? If so, what dilution, frozen or paraffin and what method of antigen retrieval |
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ANSWER: |
This is a new antibody that has only yet been tested on human.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"