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TGF alpha protein (Active) (ab9587)

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Overview

  • Product nameTGF alpha protein (Active)See all TGF alpha proteins and peptides ...
  • Protein descriptionHuman TGF alpha.
  • Expression hostE. coli

    • Properties

  • Purification notesSterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised:Reconstitute with 100ul of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Concentration information loading...
  • Additional notesThe ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is < 0.2 ng/ml, corresponding to a specific activity of > 5 x 106 units/mg.
  • Sequence notesRecombinant Human TGF-a is a 5.5 kDa protein containing 50 amino acid residues: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHA DLLA
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab9587 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab9585.

    • Protein info

    • Alternative names
        EGF like TGFEGF-like TGFETGF
        TFGATGF ATGF type 1TGF-alphaTGFATGFA_HUMANTransforming growth factor alphaTransforming growth factor alpha precursorWa1Waved 1
      see all
  • FunctionTGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
  • Tissue specificityIsoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.
  • Sequence similaritiesContains 1 EGF-like domain.
  • Cellular localizationCell membrane and Secreted > extracellular space.

    • Target information above from: UniProt accession P01135 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for TGF alpha protein (Active) (ab9587)

    ab9587 has not yet been referenced specifically in any publications.

    Publishing research using ab9587 ? Please let us know so that we can cite the reference in this datasheet

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    I was wondering if this product is approved for cell culture?

    Thank you once again for your enquiry. I am pleased to forward you some futher procedure details for testing of ab in cell culture. I hope this will be helpful to you. 1. Resuspend 3T3 cells (passage#38) in regular culture media with 10% calf se...

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    Ab9863: BATCH NUMBER 107563 ORDER NUMBER 79846 DESCRIPTION OF THE PROBLEM This recombinant protein is lyophilized. For our applications the buffer salt concentration is critical. We need to know the volume of the product at the time ...

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    Thank you for your enquiry and thank you very much for your patience. I do apologize for the delay, it some time to get this information from the originator of these peptides. For each of the products listed, included is the concentration of each pro...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"