TGF beta 1 Mouse ELISA Kit (with color giving dyes) (ab119557)
- Product nameTGF beta 1 Mouse ELISA Kit (with color giving dyes)See all TGF beta 1 kits ...
Intra-assay Sample n Mean SD CV% Overall 7.9% Overall 7.9% Inter-assay Sample n Mean SD CV% Overall 5.8%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity7.8 pg/ml
- Range31.3 pg/ml - 2000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 124% - 141% Serum 92% - 103%
- Species reactivityReacts with: Mouse
- Product overview
ab119557 TGF beta 1 Mouse ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of mouse TGF beta 1. ab119557 is for research use only and is not for diagnostic or therapeutic procedures.
TGF beta plays a critical role in cellular growth, development, differentiation, proliferation, extracellular matrix (ECM) synthesis and degradation, control of mesenchymal-epithelial interactions during embryogenesis, immune modulation, apoptosis, cell cycle progression, angiogenesis, adhesion and migration and leukocyte chemotaxis. It has both tumor suppressive and tumor promoting activities and is highly regulated at all levels. TGF beta 1 is the key mediator in the pathophysiology of tissue repair and human fibrogenesis: balance between production and degradation of type I collagen, and fibrosis and scarring in organ and tissue. TGF beta 1 promotes Treg cell differentiation resulting in IL-10/TGF beta 1 production and Th1 cell and Th2 cell suppression.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Adhesive Films 6 units Assay Buffer Concentrate (20x) 2 x 5ml Biotin-Conjugate anti-mouse TGF beta 1 polyclonal antibody 1 x 120µl Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml Hydrochloric acid 1 x 3ml Monoclonal anti-mouse TGF beta 1-coated microwell plate 1 unit Mouse TGF beta 1 Standard 2 vials Red-Dye 1 x 0.4ml Sodium hydroxide 1 x 3ml Stop Solution 1 x 15ml Streptavidin-HRP 1 x 150µl Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
- Camurati-Engelmann diseaseCEDDPD1
- LAPLatency-associated peptideTGF beta 1 protein TGF-beta-1TGFBtgfb1TGFB1_HUMANTGFbetaTransforming growth factor beta 1
Our Abpromise guarantee covers the use of ab119557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
TGF beta 1 Mouse ELISA Kit (with color giving dyes) images
Representative standard curve using ab119557.
References for TGF beta 1 Mouse ELISA Kit (with color giving dyes) (ab119557)
ab119557 has not yet been referenced specifically in any publications.