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TGF beta 1 Mouse ELISA Kit (with color giving dyes) (ab119557)

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Overview

  • Product nameTGF beta 1 Mouse ELISA Kit (with color giving dyes)See all TGF beta 1 kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 7.9%
    Overall 7.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 5.8%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    7.8 pg/ml
  • Range
    31.3 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 124% - 141%
    Serum 92% - 103%

  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab119557 TGF beta 1 Mouse ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of mouse TGF beta 1. ab119557 is for research use only and is not for diagnostic or therapeutic procedures.

    TGF beta plays a critical role in cellular growth, development, differentiation, proliferation, extracellular matrix (ECM) synthesis and degradation, control of mesenchymal-epithelial interactions during embryogenesis, immune modulation, apoptosis, cell cycle progression, angiogenesis, adhesion and migration and leukocyte chemotaxis. It has both tumor suppressive and tumor promoting activities and is highly regulated at all levels. TGF beta 1 is the key mediator in the pathophysiology of tissue repair and human fibrogenesis: balance between production and degradation of type I collagen, and fibrosis and scarring in organ and tissue. TGF beta 1 promotes Treg cell differentiation resulting in IL-10/TGF beta 1 production and Th1 cell and Th2 cell suppression.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Post-translational
    modifications    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P01137 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Camurati-Engelmann diseaseCEDDPD1
        LAPLatency-associated peptideTGF beta 1 protein TGF-beta-1TGFBtgfb1TGFB1_HUMANTGFbetaTransforming growth factor beta 1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119557 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      TGF beta 1 Mouse ELISA Kit (with color giving dyes) images

      • Sandwich ELISA - TGF beta 1 Mouse ELISA Kit (with color giving dyes) (ab119557)
        Sandwich ELISA - TGF beta 1 Mouse ELISA Kit (with color giving dyes) (ab119557)
        Representative standard curve using ab119557.

      Protocols

      References for TGF beta 1 Mouse ELISA Kit (with color giving dyes) (ab119557)

      ab119557 has not yet been referenced specifically in any publications.

      Publishing research using ab119557 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab119557 Submit a Question for ab119557

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      Votes: Highest First

      Hello,



      I would like to order this kit, but first I want to ask whether I can use this kit for also cell lysates or not. Thank you in advance.



      Best Regards,






      We do have reports concerning tissue samples for ab119557 indicating that lysis buffers containing 0.5% TritonX-100 (detergent) had no deleterious effect, but we did not test this ourselves.


      We also have not tested...

      Read More

      Dear All,
      I'm writing because a customer of ours is looking for a Mouse TGF-beta1 Kit ELISA for tissue lysate samples.

      I found your AB119557 but it is just tested for Cell culture supernatant, Serum andPlasma.

      Do you have any da...

      Read More

      Thank you for contacting us.

      The TGF beta 1 Mouse ELISA Kit (with color giving dyes) (ab119557) is the only kit we currently have which can be used to detect TGF beta 1 in mouse samples. Regrettably we have not as yet tested this kit with tissue...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"