Anti-TGF beta 1 antibody [2Ar2] (ab64715)

Thank you Jeremy for the detailed explanation of this protein. Can you tell us what is the expression pattern of this protein under the IHC staining? Is it expected to be nucleic or plasma or both?

ANSWER:

Thank you for your enquiry.

The majority of staining for TGF beta 1 will be in the extracellular space however you may see some staining of protein on its way out of the cell as its being processed.

I hope this is helpful. Please contact me again if you have any further questions.

Thanks for review our inquiry. We would like to understand from your side

Does the TGF-beta1 signals should locate in the cell membranes, plasma and nuclei in IHC assay?

ANSWER:

Thank you for your enquiry.

The protein is secreted from the cell. It is then cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members.

I hope this is helpful. Please contact me again if you have any further questions.

I brought only one vial TGF beta1, so I don’t have the same antibody with another lot. However, I found the purchase order number. The abcam product was distributed by a Dutch company called ITK diagnostics BV in Uithoorn. The purchase order number of ITK diagnostics was AM-4243781.
Kind regards,

ANSWER:

Please contact ITK diagnostic for the replacement. I cannot directly send it to you because we do not have your address details. You can discuss this case with ITK. Could you tell them to send me an email for acceptance of FOCR for you?

Thanks for review. Yes, the 50 samples were all sections of the synovial sarcoma tissue.

Normally for nucleus staining, if they were not expected they might be chance that nonspecific.



Thanks and look forward to your further support.

ANSWER:

Is there any way to explain why most of the samples, from the same tissue, using the same primary antibody and same protocol,stain as expected while some show nuclear staining?

Dear Abcam US Technical,



We have inquiry to the attached antibody that we did not notice a consistent staining data as we expected from literature. We would like to understand perhaps some information, or advice from you?

ANSWER:

Thank you for your enquiry and for providing your completed questionnaire.

You noted that a total of 50 samples were tested and about 11 sampleshad staining at the nucleus.

Were all these 50 samples of synovial sarcoma tissue sections? It is interesting thatmost would stainas expected but some wouldonly stain nuclear.

Thanks in advance for the additional information.