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Anti-TGF beta 1 antibody [2Ar2] (ab64715)

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Overview

Product name

Anti-TGF beta 1 antibody [2Ar2]
See all TGF beta 1 products (17) ...

Description

Mouse monoclonal [2Ar2] to TGF beta 1

Specificity

ab64715 neutralizes the biological activity of TGF beta 1 and TGF beta 1.2. (heterodimer of TGF beta 1 and TGF beta 2). In sandwich ELISA, less than 2% crossreactivity with TGF beta 3 and TGF beta 5 was observed and no crossreactivity was observed with other cytokines tested. With mouse RAW 264.7 cell lysates, a broad band is observed at ~45kD in Western Blot.

Tested applications

WB, ELISA, IHC-P, IHC-Fr, ICC, Neutralising, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Recombinant human TGF beta 1 and latent TGF beta 1 expressed from CHO cells.

Positive control

HT2 cells, RAW 264.7 cells, PC-3 cells

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: None
Constituents: 5% Trehalose, 40% Glycerol, PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

2Ar2

Isotype

IgG1

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TGF beta 1 antibody [2Ar2] (ab64715)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TGF beta 1 antibody [2Ar2] (ab64715) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab64715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB: Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).(The detection limit for hTGF beta 1 is ~5ng/lane under non-reducing conditions.)

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    ELISA

     ELISA: Use a concentrati...Read more →

    ELISA: Use a concentration of 2 - 8 µg/ml.(Using 100µl/well will detect ~30-2000pg/ml)

  • IHC-P: Use a concentration of 8 - 25 µg/ml.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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    IHC-Fr

     IHC-Fr: Use a concentrat...Read more →

    IHC-Fr: Use a concentration of 8 - 25 µg/ml.

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    ICC

     ICC: Use a concentration...Read more →

    ICC: Use a concentration of 8 - 25 µg/ml.

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    Neutralising

     Neut: Use a concentratio...Read more →

    Neut: Use a concentration of 0.3 - 1 µg/ml.(This dilution will neutralize 50% of the bioactivity due to 0.25ng/ml of rhTGF beta 1, using the HT2 cell line.)

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    Flow Cyt

     Flow Cyt: Use a concentr...Read more →

    Flow Cyt: Use a concentration of 25 µg/ml

Target

Function

Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.

Tissue specificity

Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.

Involvement in disease

Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.

Sequence similarities

Belongs to the TGF-beta family.

Post-translational
modifications

Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.

Cellular localization

Secreted > extracellular space > extracellular matrix.

Target information above from: UniProt accessionP01137 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Camurati Engelmann disease antibody
  • CED antibody
  • Diaphyseal dysplasia 1 progressive antibody
  • DPD1 antibody
  • LAP antibody
  • Latency-associated peptide antibody
  • TGF beta 1 antibody
  • TGF beta antibody
  • TGF beta 1 protein antibody
  • TGF-beta-1 antibody
  • TGF-beta-5 antibody
  • TGF-beta1 antibody
  • TGFB antibody
  • Tgfb-1 antibody
  • TGFB1 antibody
  • TGFB1_HUMAN antibody
  • TGFbeta antibody
  • TGFbeta1 antibody
  • Transforming Growth Factor b1 antibody
  • Transforming Growth Factor beta 1 antibody
  • Transforming growth factor beta 1a antibody
see all

Anti-TGF beta 1 antibody [2Ar2] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TGF beta 1 antibody [2Ar2] (ab64715)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TGF beta 1 antibody [2Ar2] (ab64715)

ab64715 staining TGF beta 1 in mouse liver tissue sections by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 1% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/50 in 1% BSA) at 4°C for 12 hours. An undiluted HRP-conjugated polyclonal to mouse IgG was used as secondary antibody.

This image is courtesy of an Abreview submitted by Dr Robert Domitrovic

See Abreview

References for Anti-TGF beta 1 antibody [2Ar2] (ab64715)

This product has been referenced in:

  • Maier CL & Pober JS Human placental pericytes poorly stimulate and actively regulate allogeneic CD4 T cell responses. Arterioscler Thromb Vasc Biol 31:183-9 (2011). Neutralising; Human.Read more (PubMed: 21051666) »
  • Carthy JMet al. Wnt3a Induces Myofibroblast Differentiation by Upregulating TGF-ß Signaling Through SMAD2 in a ß-Catenin-Dependent Manner. PLoS One 6:e19809 (2011). WB; Mouse.Read more (PubMed: 21611174) »

See all 5 publications for this product

Publishing research using ab64715? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"