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Product Name
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TGF beta 1 antibody [2Ar2]
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See all TGF beta 1 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [2Ar2] to TGF beta 1
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Immunogen
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Recombinant human TGF beta 1 and latent TGF beta 1 expressed from CHO cells.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab64715 neutralizes the biological activity of TGF beta 1 and TGF beta 1.2. (heterodimer of TGF beta 1 and TGF beta 2). In sandwich ELISA, less than 2% crossreactivity with TGF beta 3 and TGF beta 5 was observed and no crossreactivity was observed with other cytokines tested.
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Tested applications
(see key)
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ELISA, Flow Cyt, ICC, IHC-Fr, IHC-P, Neut, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 2 - 8 µg/ml. Using 100µl/well will detect ~30-2000pg/ml
Flow Cyt: Use at a concentration of 25 µg/ml.
ICC: Use at a concentration of 8 - 25 µg/ml.
IHC-P: Use at a concentration of 8 - 25 µg/ml.
IHC-Fr: Use at a concentration of 8 - 25 µg/ml.
Neut: Use at a concentration of 0.3 - 1 µg/ml will neutralize 50% of the bioactivity due to 0.25ng/ml of rhTGF beta 1, using the HT2 cell line.
WB: Use at a concentration of 1 - 2 µg/ml. The detection limit for hTGF beta 1 is ~5ng/lane under non-reducing conditions. Predicted molecular weight: 25 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HT2 cell line.
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Cellular localization
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Secreted
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> TGF
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Signal Transduction >> Growth Factors/Hormones >> TGF
Cardiovascular >> Angiogenesis >> Growth Factors >> TGF
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Relevance
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TGF beta 1 (Transforming Growth Factor Beta 1) is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non neoplastic tissues, release transforming growth factors; and essentially all cells possess a specific TGF beta 1 receptor. The multimodal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function.
The C-terminal 112 amino acids constitute the mature protein.
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TGF beta 1 antibody [2Ar2] (ab64715) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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2Ar2
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 5% Trehalose, 40% Glycerol, PBS, pH 7.2 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TGF beta 1 antibody [2Ar2] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to TGF beta 1
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TGF beta 1 antibody [2Ar2] - more information
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