Products:Cardiovascular >> Angiogenesis >> Growth Factors >> TGF
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab27969? Please let us know so that we can cite the reference in this datasheet
ab27969 has been referenced in 8 publications.
Publishing research using ab27969? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab27969 staining TGF beta 1 in rat kidney tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 2% BSA for 2 hours at 25ºC followed by incubation with the primary antibody, at a 1/200 dilution, for 9 hours at 4ºC. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 594 (red) used at a 1/500 dilution.
Image courtesy of an anonymous Abreview.
ab 27969 staining TGF beta 1 in human 293ft cells by Immunocytochemistry/ Immunofluorescence.The 293 FT cells were cultured for 3 days then fixed with 3.7% formaldehyde for 10 minutes and blocked with 5% BSA in PBS for 1 hour at +4ºC. The cells were incubated with the primary antibody at 1/200 dilution overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 555 Goat anti-Rabbit IgG used at a dilution of 1/200.
Image courtesy of an anonymous Abreview.
3
Call 01223 696 000 or contact us