TGF beta 1 antibody [TB21] (ab27969)

Product Name 

TGF beta 1 antibody [TB21]

Product type 

Primary antibodies

Description 

Mouse monoclonal [TB21] to TGF beta 1

Immunogen 

Human TGF Beta 1 from platelets.

Reacts with 
(species key)

Hu, Rat, Shp

Specificity 

This antibody recognises natural and recombinant TGF beta 1. It reacts with both dimeric and monomeric natural forms under reducing and non reducing conditions.

Tested applications 
(see key)

ELISA, ICC/IF, IHC-Fr, IHC-P, Neut, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: 1/10 (PMID 18787407). Protein digestion pretreatment is not required. Paraformaldehyde fixation is recommended.
Neut: Use at an assay dependent dilution (See PubMed: 8237223)
WB: 1/2000 (see Abreview).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human breast carcinoma.

Cellular localization 

Secreted

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> TGF
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Signal Transduction >> Growth Factors/Hormones >> TGF
Cardiovascular >> Angiogenesis >> Growth Factors >> TGF

Relevance 

TGF beta 1 (Transforming Growth Factor Beta 1) is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non neoplastic tissues, release transforming growth factors; and essentially all cells possess a specific TGF beta 1 receptor. The multimodal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function.
The C-terminal 112 amino acids constitute the mature protein.

This clone (TB21) demonstrates neutralising activity against TGF beta 1 in cell proliferation assays. Removal of sodium azide is recommended prior to use in functional assays.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

TB21

Myeloma 

Sp2/0-Ag14

Isotype 

IgG1

Light chain type 

kappa

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.09% Sodium Azide.
Constituents: PBS.

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

References for TGF beta 1 antibody [TB21] (ab27969)

  This product has been used in: (two most recent references)

Ares-Carrasco S et al. Myocardial fibrosis and apoptosis, but not inflammation, are present in long-term experimental diabetes. Am J Physiol Heart Circ Physiol 297:H2109-19 (2009). WB; Rat. PubMed: 19820199

Zlobec I et al. Node-negative colorectal cancer at high risk of distant metastasis identified by combined analysis of lymph node status, vascular invasion, and Raf-1 kinase inhibitor protein expression. Clin Cancer Res 14:143-8 (2008). IHC-P; Human. PubMed: 18172264

See all 5 publication references for this product.


If you publish research using ab27969 please let us know so that we can cite the reference on this datasheet.

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