Overview

  • Product nameAnti-TGF beta 1 antibody [TB21]See all TGF beta 1 primary antibodies ...
  • Description
    Mouse monoclonal [TB21] to TGF beta 1
  • SpecificityThis antibody recognises natural and recombinant TGF beta 1. It reacts with both dimeric and monomeric natural forms under reducing and non reducing conditions.
  • Tested applicationsIHC-Fr, IHC-P, ELISA, ICC/IF, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Sheep, Human, Pig
    Predicted to work with: Rat
  • Immunogen

    Human TGF Beta 1 from human platelets.

  • Positive control
  • General notes

    We have mixed feedback from customers about the Rat specificity so the Rat species has been moved to predicted as we can't guarantee it. The antibody has been successfully used with Rat samples in publication PMID 22471627 and PMID 19820199 though.

     

    This clone (TB21) demonstrates neutralising activity against TGF beta 1 in cell proliferation assays. Removal of sodium azide is recommended prior to use in functional assays.

Properties

Applications

Our Abpromise guarantee covers the use of ab27969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
Neutralising Use at an assay dependent dilution. PubMed: 8237223
WB 1/2000. See Abreview. We recommend using BSA in blocking solution.

Target

  • FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Post-translational
    modifications
    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Camurati Engelmann disease antibody
    • CED antibody
    • Diaphyseal dysplasia 1 progressive antibody
    • DPD1 antibody
    • LAP antibody
    • Latency-associated peptide antibody
    • Prepro transforming growth factor beta 1 antibody
    • TGF beta 1 antibody
    • TGF beta antibody
    • TGF beta 1 protein antibody
    • TGF-beta 1 protein antibody
    • TGF-beta-1 antibody
    • TGF-beta-5 antibody
    • TGF-beta1 antibody
    • TGFB 1 antibody
    • TGFB antibody
    • Tgfb-1 antibody
    • TGFB1 antibody
    • TGFB1_HUMAN antibody
    • TGFbeta antibody
    • TGFbeta1 antibody
    • Transforming Growth Factor b1 antibody
    • Transforming Growth Factor beta 1 antibody
    • Transforming growth factor beta 1a antibody
    • transforming growth factor beta-1 antibody
    • transforming growth factor, beta 1 antibody
    see all

Anti-TGF beta 1 antibody [TB21] images

  • ab27969 staining TGF beta 1 in rat kidney tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 2% BSA for 2 hours at 25°C followed by incubation with the primary antibody, at a 1/200 dilution, for 9 hours at 4°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 594 (red) used at a 1/500 dilution.

    See Abreview

  • ab 27969 staining TGF beta 1 in human 293ft cells by Immunocytochemistry/ Immunofluorescence.
    The 293 FT cells were cultured for 3 days then fixed with 3.7% formaldehyde for 10 minutes and blocked with 5% BSA in PBS for 1 hour at +4°C. The cells were incubated with the primary antibody at 1/200 dilution overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 555 Goat anti-Rabbit IgG used at a dilution of 1/200.
  • ab27969 staining TGF beta 1 in Human ureter tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/150) for 16 hours at 4°C. A Cy2®-conjugated Goat anti-mouse polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-TGF beta 1 antibody [TB21] (ab27969)

This product has been referenced in:
  • Gay-Jordi G  et al. Losartan prevents heart fibrosis induced by long-term intensive exercise in an animal model. PLoS One 8:e55427 (2013). WB ; Rat . Read more (PubMed: 23383330) »
  • Gaber N  et al. Fetal reprogramming and senescence in hypoplastic left heart syndrome and in human pluripotent stem cells during cardiac differentiation. Am J Pathol 183:720-34 (2013). IHC-P . Read more (PubMed: 23871585) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (Ureter)
Specification Ureter
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Ureter)
Specification Ureter
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Ms. Beate Thode

Verified customer

Submitted Mar 14 2013

Vielen Dank für Ihre Anfrage.

Ich freue mich ihnen mitteilen zu koennen dass wir fuer den gesamten November unsere Aktion 4 fuer 3 laufen haben. Bei der Bestellung von 4 Produkten bekommen Sie das guenstigste Produkt kostenfrei.

...

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Thank you for getting back to me with that information.

I am not entirely sure why you have lost the staining. Could it be that the level of TGF beta 1 is quite low in your samples? I have found reference to it being quite low in normal rat b...

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Thank you for getting back to me with that information.

I am sorry to hear that you are still having difficulties with the TGF beta 1 staining. Since your initial complaint we have retested both the lots of ab27969 you used and have not been ...

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Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Je me permets de vous transférer le numéro de commande de remplacemen...

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Merci de votre réponse et d'avoir pris en compte mes suggestions. Puisque dans le contrôle positive vous ne voyez pas non plus la bande spécifique mais les autres bandes, je dois conclure que l'anticorps est endommagé. Je sui...

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Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés. Je suis désolée que vous avez eu des difficultés à obtenir de bons résultats cet anticorps.

Comme vous savez, no...

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Thank you very much for contacting us.

I have looked at the review you have been attempting to submit and I have a few questions.

1. The review xxxxx is for the antibody anti-Osteopontin antibody ab11503, not the anti-TGF beta 1 ant...

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Je suis désolée d'apprendre que ab27969 ne vous donne pas de bons résultats en Western blot.

Veuillez trouver ci-jointl questionnaire discuté au téléphone qui nous permet de regrouper le plus d'infor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"