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Human TGF Beta 1 from human platelets.
We have mixed feedback from customers about the Rat specificity so the Rat species has been moved to predicted as we can't guarantee it. The antibody has been successfully used with Rat samples in publication PMID 22471627 and PMID 19820199 though.
This clone (TB21) demonstrates neutralising activity against TGF beta 1 in cell proliferation assays. Removal of sodium azide is recommended prior to use in functional assays.
Our Abpromise guarantee covers the use of ab27969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
|Neutralising||Use at an assay dependent concentration. PubMed: 8237223|
See Abreview. We recommend using BSA in blocking solution. Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"