Anti-TGF beta 1 antibody (ab46780)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab46780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA
  • Application notesELISA: 1/2000 - 1/5000.
    WB: 1/2000 - 1/5000. Predicted molecular weight: 47 kDa for the unprocessed precursor; 25 kDa for the mature protein; 13KDa for degraded or cleaved protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
    • Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
    • Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
    • Sequence similaritiesBelongs to the TGF-beta family.
    • Post-translational
      modifications
      Glycosylated.
      The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Camurati Engelmann disease antibody
      • CED antibody
      • Diaphyseal dysplasia 1 progressive antibody
      • DPD1 antibody
      • LAP antibody
      • Latency-associated peptide antibody
      • Prepro transforming growth factor beta 1 antibody
      • TGF beta 1 antibody
      • TGF beta antibody
      • TGF beta 1 protein antibody
      • TGF-beta 1 protein antibody
      • TGF-beta-1 antibody
      • TGF-beta-5 antibody
      • TGF-beta1 antibody
      • TGFB 1 antibody
      • TGFB antibody
      • Tgfb-1 antibody
      • TGFB1 antibody
      • TGFB1_HUMAN antibody
      • TGFbeta antibody
      • TGFbeta1 antibody
      • Transforming Growth Factor b1 antibody
      • Transforming Growth Factor beta 1 antibody
      • Transforming growth factor beta 1a antibody
      • transforming growth factor beta-1 antibody
      • transforming growth factor, beta 1 antibody
      see all

    Anti-TGF beta 1 antibody images

    • ICC/IF image of ab46780 stained T24/83 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46780 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- chicken (ab150173) IgY (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • All lanes : Anti-TGF beta 1 antibody (ab46780) at 1/2000 dilution (ab50036, 0.1ug)

      Lane 1 : As above
      Lane 2 : As above

      Secondary
      Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

    References for Anti-TGF beta 1 antibody (ab46780)

    ab46780 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    We do not have mouse monoclonal antibodies to the target you are requested. However we do have following antibodies available;

    ab9758; http://www.abcam.com/TGF-beta-1-antibody-ab9758.html

    ab46780;...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"