Anti-TGF beta 1 antibody (ab92486)
Key features and details
- Rabbit polyclonal to TGF beta 1
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-TGF beta 1 antibody
See all TGF beta 1 primary antibodies -
Description
Rabbit polyclonal to TGF beta 1 -
Host species
Rabbit -
Specificity
Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
ab92486 detects the mature secreted form of the protein at 13 kDa. Our internal testing data suggests that this does not detect the full length protein at 44kDa. As cell lysates are unsuitable for detection of the mature form, tissue lysates should be used when using this antibody.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human TGF beta 1. Surrounding amino acid 374.
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Positive control
- WB: Human spleen and kidney tissue lysates.
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 5559 Anti-TGF-ß1 Polyclonal Antibody. 5559-100 is the same size as the 100 µg size of ab92486.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.5% BSA, 30% Glycerol (glycerin, glycerine), 0.15% EDTA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 44 kDa.
Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. |
Notes |
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WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 44 kDa. Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. |
Target
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Function
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
Tissue specificity
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
Involvement in disease
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
Sequence similarities
Belongs to the TGF-beta family. -
Post-translational
modificationsGlycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 7040 Human
- Omim: 190180 Human
- SwissProt: P01137 Human
- Unigene: 645227 Human
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Alternative names
- Cartilage-inducing factor antibody
- CED antibody
- Differentiation inhibiting factor antibody
see all
Images
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All lanes : Anti-TGF beta 1 antibody (ab92486)
Lane 1 : Human recombinant TGF-ß1 at 0.0005 µg
Lane 2 : Human kidney tissue at 50 µg
Lane 3 : Human spleen tissue at 50 µg
Predicted band size: 44 kDa -
All lanes : Anti-TGF beta 1 antibody (ab92486) at 5 µg/ml
Lane 1 : Human spleen tissue lysate
Lane 2 : Human kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab92486 observed at 15 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab92486 was shown to react with TGF beta 1 in western blot. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab92486 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at 5 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (596)
ab92486 has been referenced in 596 publications.
- Yamane S et al. The role of thromboxane prostanoid receptor signaling in gastric ulcer healing. Int J Exp Pathol 103:4-12 (2022). PubMed: 34655121
- Bi L et al. Pirfenidone Attenuates Renal Tubulointerstitial Fibrosis through Inhibiting miR-21. Nephron 146:110-120 (2022). PubMed: 34724669
- Wu J et al. Rubus chingii Hu. unripe fruits extract ameliorates carbon tetrachloride-induced liver fibrosis and improves the associated gut microbiota imbalance. Chin Med 17:56 (2022). PubMed: 35549741
- Mancinelli R et al. The Effects of Taurocholic Acid on Biliary Damage and Liver Fibrosis Are Mediated by Calcitonin-Gene-Related Peptide Signaling. Cells 11:N/A (2022). PubMed: 35563897
- Wang J et al. Empagliflozin Ameliorates Diabetic Cardiomyopathy via Attenuating Oxidative Stress and Improving Mitochondrial Function. Oxid Med Cell Longev 2022:1122494 (2022). PubMed: 35585884