Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cardiovascular >> Angiogenesis >> Growth Factors >> TGF
Overview
Product name
Anti-TGF beta 1 antibody
See all TGF beta 1 products (17) ...
Description
Rabbit polyclonal to TGF beta 1
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Sheep, Rabbit, Horse, Guinea pig, Hamster, Cow, Pig
Does not react with
Dog
Immunogen
Positive control
recombinant human TGFß1
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> TGF
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Signal Transduction >> Growth Factors/Hormones >> TGF
Cardiovascular >> Angiogenesis >> Growth Factors >> TGF
-
Western blot - Anti-TGF beta 1 antibody (ab9758)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/500.Detects a band of approximately 25 , 12 kDa.
Target
Function
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
Tissue specificity
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
Involvement in disease
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
Sequence similarities
Belongs to the TGF-beta family.
Post-translational
modifications
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP01137
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Camurati Engelmann disease antibody
- CED antibody
- Diaphyseal dysplasia 1 progressive antibody
see all
Database links
- Entrez Gene: 282089 Cow
- Entrez Gene: 7040 Human
- Entrez Gene: 21803 Mouse
- Entrez Gene: 397078 Pig
- Omim: 190180 Human
- SwissProt: P01137 Human
- SwissProt: P04202 Mouse
- SwissProt: P07200 Pig
see all
Anti-TGF beta 1 antibody images:
Western blot - Anti-TGF beta 1 antibody (ab9758)
Western blot analysis using ab9758 at 1/200 to detect human TGFß1. Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). Comparison to molecular weight markers (not shown) was used to estimate the indicated molecular weights. TGF beta 1 is a 25kD homodimer composed of two 12.5kD subunits joined by disulfide bonds. Therefor a 25kD band is seen in the non-reducing lane and a 12kD band in the reducing lane.
References for Anti-TGF beta 1 antibody (ab9758)
This product has been referenced in:
- Nicola Tet al. Hypoxia-induced inhibition of lung development is attenuated by the peroxisome proliferator-activated receptor-{gamma} agonist rosiglitazone. Am J Physiol Lung Cell Mol Physiol 301:L125-34 (2011). WB; Mouse.Read more (PubMed: 21531777) »
- Joner Met al. Pioglitazone inhibits in-stent restenosis in atherosclerotic rabbits by targeting transforming growth factor-beta and MCP-1. Arterioscler Thromb Vasc Biol 27:182-9 (2007).Read more (PubMed: 17068283) »
See all 3 publications for this product
Publishing research using ab9758? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"