Overview
- Product nameAnti-TGF beta 1 antibodySee all TGF beta 1 primary antibodies ...
- DescriptionRabbit polyclonal to TGF beta 1
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Guinea pig, Hamster, Cow, PigDoes not react with
Dog - Immunogen
Synthetic peptide: FSSTEKN (Human)
- Positive controlrecombinant human TGFß1
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage buffer0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
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Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab9758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/100 - 1/500. In reduced samples, the expected bands are 12 kDa (mature protein) and 44 kDa (unprocessed protein). Expected band at 25 kDa in non-reduced samples (homodimer of mature protein). Glycosylations may also cause unprocessed protein to run a few kDa higher (around 50 kDa). |
Target
- FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
- Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
- Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
- Sequence similaritiesBelongs to the TGF-beta family.
- Post-translational
modificationsGlycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. - Cellular localizationSecreted > extracellular space > extracellular matrix.
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Database links
- Entrez Gene: 7040 Human
- Entrez Gene: 21803 Mouse
- Omim: 190180 Human
- SwissProt: P01137 Human
- SwissProt: P04202 Mouse
- Unigene: 645227 Human
- Unigene: 248380 Mouse
Target information above from: UniProt accession
P01137
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Camurati Engelmann disease antibodyCED antibodyDiaphyseal dysplasia 1 progressive antibody
- DPD1 antibodyLAP antibodyLatency-associated peptide antibodyTGF beta 1 antibodyTGF beta antibodyTGF beta 1 protein antibodyTGF-beta 1 protein antibodyTGF-beta-1 antibodyTGF-beta-5 antibodyTGF-beta1 antibodyTGFB 1 antibodyTGFB antibodyTgfb-1 antibodyTGFB1 antibodyTGFB1_HUMAN antibodyTGFbeta antibodyTGFbeta1 antibodyTransforming Growth Factor b1 antibodyTransforming Growth Factor beta 1 antibodyTransforming growth factor beta 1a antibodytransforming growth factor beta-1 antibodytransforming growth factor, beta 1 antibody
see all
Anti-TGF beta 1 antibody images
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Western blot - Anti-TGF beta 1 antibody (ab9758)Western blot analysis using ab9758 at 1/200 to detect human TGFß1. Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). Comparison to molecular weight markers (not shown) was used to estimate the indicated molecular weights. TGF beta 1 is a 25kD homodimer composed of two 12.5kD subunits joined by disulfide bonds. Therefor a 25kD band is seen in the non-reducing lane and a 12kD band in the reducing lane.
References for Anti-TGF beta 1 antibody (ab9758)
This product has been referenced in:
- Quarto N et al. Skeletogenic phenotype of human Marfan embryonic stem cells faithfully phenocopied by patient-specific induced-pluripotent stem cells. Proc Natl Acad Sci U S A 109:215-20 (2012). Human . Read more (PubMed: 22178754) »
- Nicola T et al. Hypoxia-induced inhibition of lung development is attenuated by the peroxisome proliferator-activated receptor-{gamma} agonist rosiglitazone. Am J Physiol Lung Cell Mol Physiol 301:L125-34 (2011). WB ; Mouse . Read more (PubMed: 21531777) »
