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- TGF beta 1 antibody (ab9758)
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Product Name
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TGF beta 1 antibody
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See all TGF beta 1 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TGF beta 1
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Immunogen
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Synthetic peptide: FSSTEKN (Human)
BLAST 'FSSTEKN-C' with  or 
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Reacts with
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Hu
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Does not react with
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Dog
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Predicted to react
(species key)
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Ms, Cow, Gpig, Hm, Hrs, Pig, Rb, Shp
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000 - 1/5000. Use at an assay dependent dilution against the immunizing peptide
WB: 1/100 - 1/500. Detects a band of approximately 25 & 12 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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recombinant human TGFß1
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Cellular localization
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Secreted
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> TGF
Stem Cells >> Signaling Pathways >> TGF beta >> Secreted
Signal Transduction >> Growth Factors/Hormones >> TGF
Cardiovascular >> Angiogenesis >> Growth Factors >> TGF
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Relevance
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TGF beta 1 (Transforming Growth Factor Beta 1) is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non neoplastic tissues, release transforming growth factors; and essentially all cells possess a specific TGF beta 1 receptor. The multimodal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function.
The C-terminal 112 amino acids constitute the mature protein.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.530 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TGF beta 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TGF beta 1 antibody images, references, products related to ab9758 and other tools.
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TGF beta 1 antibody images:
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 - TGF beta 1 antibody (ab9758)
Western blot analysis using ab9758 at 1/200 to detect human TGFß1.
Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2).
Comparison to molecular weight markers (not shown) was used to estimate the indicated molecular weights.
TGF beta 1 is a 25kD homodimer composed of two 12.5kD subunits joined by disulfide bonds. Therefor a 25kD band is seen in the non-reducing lane and a 12kD band in the reducing lane.
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References for TGF beta 1 antibody (ab9758)
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This product has been used in: (two most recent references)
Joner M et al. Pioglitazone inhibits in-stent restenosis in atherosclerotic rabbits by targeting transforming growth factor-beta and MCP-1. Arterioscler Thromb Vasc Biol 27:182-9 (2007). PubMed: 17068283
Hoek KS et al. Metastatic potential of melanomas defined by specific gene expression profiles with no BRAF signature. Pigment Cell Res 19:290-302 (2006). PubMed: 16827748
If you publish research using ab9758 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to TGF beta 1
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TGF beta 1 antibody - more information
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Customer reviews (feedback) regarding TGF beta 1 antibody |
Customer FAQs regarding TGF beta 1 antibody |
Protocols for TGF beta 1 antibody |
Price and availability of products related to TGF beta 1 antibody |
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TGF beta 1 antibody - related products:
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TGF beta 1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about TGF beta 1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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