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Overview

  • Product nameTGF beta 2 protein (Active)See all TGF beta 2 proteins and peptides ...
  • Protein descriptionRecombinant full length Human TGF beta 2 (amino acids 303-414) expressed in A293 cells; 25 kDa; composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond (SwissProt P61812).
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesPurity is greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Biological activityBiological Activity : Determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The ED50 was found to be = 0.2 ng/ml, corresponding to a specific activity of = 5 x 106 units/mg.
  • FormLyophilised:Reconstituted TGF beta 2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°CThis product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Concentration information loading...
  • Sequence notesALDAAYCFRN VQDNCCLRPL YIDFKRDLGW KWIHEPKGYN ANFCAGACPY LWSSDTQHSR VLSLYNTINP EASASPCCVS QDLEPLTILY YIGKTPKIEQ LSNMIVKSCK CS
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab84070 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent concentration. Ab84070 can be used as a WB positive control in conjunction with ab66045.

    Protein info

    • Alternative names
        BSC-1 cell growth inhibitorBSC1 cell growth inhibitorCetermin
        G-TSFGlioblastoma-derived T-cell suppressor factorGTSFMGC116892MGFMilk growth factorPolyerginTGF-beta-2TGF-beta2TGFB2TGFB2_HUMANTransforming growth factor beta 2Transforming growth factor beta-2
      see all
  • FunctionTGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
  • Involvement in diseaseNote=A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with HDAC9.
    Defects in TGFB2 are the cause of Loeys-Dietz syndrome 4 (LDS4) [MIM:614816]. An aortic aneurysm syndrome with widespread systemic involvement. LDS4 is characterized by arterial tortuosity, aortic dissection, intracranial aneurysm and subarachnoid hemorrhage, hypertelorism, bifid uvula, pectus deformity, bicuspid aortic valve, arachnodactyly, scoliosis, foot deformities, dural ectasia, joint hyperflexibility, and thin skin with easy bruising and striae.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P61812 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    TGF beta 2 protein (Active) images

    • Western blot
      Western blot
      Anti-TGF beta 2 antibody (ab66045) at 1 µg/ml + TGF beta 2 protein (Active) (ab84070) at 0.1 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 1 minute

    References for TGF beta 2 protein (Active) (ab84070)

    This product has been referenced in:
    • Tran DQ  et al. GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells. Proc Natl Acad Sci U S A 106:13445-50 (2009). Read more (PubMed: 19651619) »
    • O'Riordan E  et al. Chronic NOS inhibition actuates endothelial-mesenchymal transformation. Am J Physiol Heart Circ Physiol 292:H285-94 (2007). Read more (PubMed: 16963618) »

    See all 2 Publications for this product

    Publishing research using ab84070 ? Please let us know so that we can cite the reference in this datasheet

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    Inquiry: How to do reconstitution of ab50036 and ab84070. Thanks.

    Thank you for contacting us. Reconstitution instructions for the proteins are below.

    Centrifuge the vial prior to opening. Reconstitute in 10mM Citric Acid, pH 3.0 to a
    concentration of 0.1-1.0 mg/ml. Do not vortex. For
    extended storage, ...

    Read More

    How to reconstitute product ab84070?

    Thank you for contacting us.

    I can confirm that the product can be reconstituted with sterile water at conc. 0.1-1mg/ml.

    Please note the concentration will depend on the amount of water you add. e.g. if 1ml is added the conc. will be 5ug...

    Read More

    Abcam product ab66045: anti TGF beta 2 antibody.
    Figure 2 of the product datasheet shows a Western blot for Anti-TGF-B2 antibody; yet, it does not indicate the species or tissue used. Also, the figure for the Western blot depicts one band at 13-kDa a...

    Read More

    Thank you for contacting Abcam regarding ab66045.


    I am sorry that the caption describing the WB image was confusing. Please allow me to clarify:


    The image represents a WB in which the antibody ab66045 was used to detect the...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"