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Overview

  • Product nameTGF beta 2 protein (Active)See all TGF beta 2 proteins and peptides ...
  • Protein descriptionRecombinant full length protein (Human) expressed using (BTI-Tn-5B1-4) cells
  • Expression hostBaculovirus infected insect (BTI-TN-5B1-4) cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesSterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • FormLyophilised:Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Concentration information loading...
  • Additional notesThe ED50, determined by hTGF-b2 ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells, is < 0.2ng/ml, corresponding to a specific activity of > 5 x 106 units/mg. Expression System = Baculovirus infected High-5 cells.
  • Sequence noteshTGF-b2 (Human Transforming Growth Factor beta 2) is a 25.0 kDa protein with each subunit containing 112 amino acid residues: ALDAAYCFRN VQDNCCLRPL YIDFKRDLGW KWIHEPKGYN ANFCAG ACPY LWSSDTQHSRVLSLYNTINP EASASPCCVS QDL EPLTILY YIGKTPKIEQ LSNMIVKSCK CS
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab629 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab167655.

    • Protein info

    • Alternative names
        BSC 1 cell growth inhibitorBSC-1 cell growth inhibitorBSC1 cell growth inhibitor
        CeterminG TSFG-TSFGlioblastoma derived T cell suppressorGlioblastoma derived T cell suppressor factorGlioblastoma-derived T-cell suppressor factorGTSFMGC116892MGFMilk growth factorPolyerginTGF B2TGF beta2TGF-beta-2TGF-beta2TGFB 2TGFB2TGFB2_HUMANTransforming growth factor beta 2Transforming growth factor beta-2
      see all
  • FunctionTGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
  • Involvement in diseaseNote=A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with HDAC9.
    Defects in TGFB2 are the cause of Loeys-Dietz syndrome 4 (LDS4) [MIM:614816]. An aortic aneurysm syndrome with widespread systemic involvement. LDS4 is characterized by arterial tortuosity, aortic dissection, intracranial aneurysm and subarachnoid hemorrhage, hypertelorism, bifid uvula, pectus deformity, bicuspid aortic valve, arachnodactyly, scoliosis, foot deformities, dural ectasia, joint hyperflexibility, and thin skin with easy bruising and striae.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P61812 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for TGF beta 2 protein (Active) (ab629)

    This product has been referenced in:
    • Titeux M  et al. Recessive dystrophic epidermolysis bullosa caused by COL7A1 hemizygosity and a missense mutation with complex effects on splicing. Hum Mutat 27:291-2 (2006). Read more (PubMed: 16470588) »

    See 1 Publication for this product

    Publishing research using ab629 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab629 Submit a Question for ab629

    Displaying 1 - 4 of 4 results for Abreviews and Q&A

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    Dear Sirs,
    Could you tel me if product listed in your catalog ab 629 TGF beta 2 protein is the active or inactive form of TGF-b?
    Thank you

    Thank you for your enquiry and your interest in our products.

    I can confirm that ab629 a recombinant full length protein (Human) expressed using (BTI-Tn-5B1-4) cells and it has been tested un functional studies so it is the active form of TGF be...

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    What are the reconstitution instructions for this protein?

    Thank you for your enquiry. Centrifuge the vial prior to opening. Reconstituting in water to a concentration of 50 µg/ml (e.g., 5 µg/100µl) will yield a solution of 1 mM citrate buffer, pH 3.5. This solution can then be stored at 4C for 1 month. It is...

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    I am interested on using TGF-beta1 protein, but as far as I have been able to look for, your company only has recombinant proteins of TGF-alpha, TGF-beta2 and -beta3. Is it true? Do you have information of a suitable source of TGF-beta1? Thank you for you...

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    Thank you for your enquiry. Yes, we currently do not carry TGF-beta 1 protein and at this time we only have TGF alpha, TGF beta 2, and TGF beta 3 proteins. I'm not who else may have TGF-beta 1 protein, but you can click on the link to "The World's An...

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    I want to know in which buffer you suggest I should reconstitute the recombinant TGFb. Also, what is the concentration I should use for the aliquots (is there a concentration under which the product becomes unstable).

    Thank you for your enquiry. We would advise you initially reconstitute in water to a concentration of 50ug/ml. You can then aliquot this solution and should not have any problems. For further dilutions, we recommend PBS containing 2mg/ml of albumin. W...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"