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TGF beta 3 protein (Active) (ab52313)

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Overview

  • Product nameTGF beta 3 protein (Active)
  • Protein descriptionRecombinant Human TGF-ß3, 25 kDa protein with each subunit containing 112 amino acid residues.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notes>99% by SDS-PAGE and HPLC analyses
  • Biological activityTGF beta 3 is fully biologically active when compared to standard. The ED50 determined by cytotoxicity assay using the world health organization standard 98/608 as a direct comparison is <0.05 ng/ml, corresponding to a specific activity of >2 x 108 units/mg.
  • FormLiquid
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 20% Ethanol and 0.1% Acetate.
    Endotoxin level is <0.1 ng per µg.
  • Concentration information loading...
  • Additional notesThis protein solution should be stored at 4°C, stable for at least 3 months. For long term storage, it is recommended to add a carrier protein (0.1% HAS or BSA).
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab52313 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration. Ab52313 can be used as a WB positive control in conjunction with ab15537.
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        ARVDFLJ16571MGC105479
        MGC118722TGF beta 3TGF beta3TGF-beta-3TGFB 3Tgfb3TGFB3_HUMANtransforming growth factor beta 3Transforming growth factor beta-3
      see all
  • FunctionInvolved in embryogenesis and cell differentiation.
  • Involvement in diseaseDefects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P10600 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    TGF beta 3 protein (Active) images

    • Western blot - Anti-TGF beta 3 antibody (ab15537)
      Western blot - Anti-TGF beta 3 antibody (ab15537)
      Anti-TGF beta 3 antibody (ab15537) at 1/200 dilution + TGF beta 3 protein (Active) (ab52313) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 30 seconds

    References for TGF beta 3 protein (Active) (ab52313)

    ab52313 has not yet been referenced specifically in any publications.

    Publishing research using ab52313 ? Please let us know so that we can cite the reference in this datasheet

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    Could you please let me know if this code can be used in cell culture? Thanks in advance

    Thank you for your email. This product is suitable for cell culture experiments. Please let me know if you have further questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"