Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab31013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
Flow Cyt Use at an assay dependent dilution.
IP Use at 1 µg/mg of lysate.
WB Use at an assay dependent dilution. Predicted molecular weight: 56 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
IHC-Fr Use at an assay dependent dilution. PubMed: 20223936

Target

  • FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta.
  • Tissue specificityFound in all tissues examined, most abundant in placenta and least abundant in brain and heart.
  • Involvement in diseaseDefects in TGFBR1 are the cause of Loeys-Dietz syndrome type 1A (LDS1A) [MIM:609192]; also known as Furlong syndrome or Loeys-Dietz aortic aneurysm syndrome (LDAS). LDS1 is an aortic aneurysm syndrome with widespread systemic involvement. The disorder is characterized by arterial tortuosity and aneurysms, craniosynostosis, hypertelorism, and bifid uvula or cleft palate. Other findings include exotropy, micrognathia and retrognathia, structural brain abnormalities, intellectual deficit, congenital heart disease, translucent skin, joint hyperlaxity and aneurysm with dissection throughout the arterial tree.
    Defects in TGFBR1 are the cause of Loeys-Dietz syndrome type 2A (LDS2A) [MIM:608967]. LDS2 is an aortic aneurysm syndrome with widespread systemic involvement. Physical findings include prominent joint laxity, easy bruising, wide and atrophic scars, velvety and translucent skin with easily visible veins, spontaneous rupture of the spleen or bowel, diffuse arterial aneurysms and dissections, and catastrophic complications of pregnancy, including rupture of the gravid uterus and the arteries, either during pregnancy or in the immediate postpartum period. LDS2 is characterized by the absence of craniofacial abnormalities with the exception of bifid uvula that can be present in some patients.
    Defects in TGFBR1 are the cause of aortic aneurysm familial thoracic type 5 (AAT5) [MIM:608967]. Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated at basal levels in the absence of ligand binding. Activated by multiple phosphorylation, mainly in the GS region.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAT 5 antibody
    • AAT5 antibody
    • Activin A receptor type II like kinase 53kDa antibody
    • Activin A receptor type II like kinase, 53kD antibody
    • Activin A receptor type II like protein kinase of 53kD antibody
    • activin A receptor type II-like kinase, 53kDa antibody
    • activin A receptor type II-like protein kinase of 53kD antibody
    • Activin receptor like kinase 5 antibody
    • Activin receptor-like kinase 5 antibody
    • ACVRLK 4 antibody
    • ACVRLK4 antibody
    • ALK 5 antibody
    • ALK-5 antibody
    • ALK5 antibody
    • LDS1A antibody
    • LDS2A antibody
    • MSSE antibody
    • Serine/threonine protein kinase receptor R4 antibody
    • Serine/threonine-protein kinase receptor R4 antibody
    • SKR 4 antibody
    • SKR4 antibody
    • TbetaR I antibody
    • TbetaR-I antibody
    • TGF beta receptor type 1 antibody
    • TGF beta receptor type I antibody
    • TGF beta type I receptor antibody
    • TGF-beta receptor type I antibody
    • TGF-beta receptor type-1 antibody
    • TGF-beta type I receptor antibody
    • TGFBR 1 antibody
    • TGFBR1 antibody
    • TGFBR1 protein antibody
    • TGFR 1 antibody
    • TGFR-1 antibody
    • TGFR1 antibody
    • TGFR1_HUMAN antibody
    • Transforming growth factor beta receptor 1 antibody
    • Transforming growth factor beta receptor I (activin A receptor type II like kinase, 53kD) antibody
    • Transforming growth factor beta receptor I antibody
    • transforming growth factor, beta receptor 1 antibody
    • transforming growth factor, beta receptor I (activin A receptor type II-like kinase, 53kD) antibody
    • Transforming growth factor-beta receptor type I antibody
    see all

Anti-TGF beta Receptor I antibody images

  • ab31013 at 1/50 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed in citrate buffer. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

    Intense staining of rat heart valve was seen in fresh cut sections compared to weaker staining in those that had been cut 2 weeks previously. Primate placenta was used as a positive control as Abcam recommends - we saw a similar staining pattern to image already on their product page (i.e. clear positive staining was confirmed in the placental outer trophoblast layers). Negative controls (substitution of primary for buffer and suitable isotype) were clear.

    See Abreview

  • Anti-TGF beta Receptor I antibody (ab31013) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • Anti-TGF beta Receptor I antibody (ab31013) at 1/1000 dilution (for 18 hours at 4°C) + Mouse intestine whole tissue lysate at 15 µg

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/8000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-TGF beta Receptor I antibody (ab31013) at 1/750 dilution (for 16 hours at 4°C) + Rat intestine whole tissue lysate at 20 µg

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab31013 staining the TGF beta receptor I in Human intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hour at 18°C; antigen retrieval was by heat mediation with citric acid (pH 5.5). Samples were incubated with primary antibody (1/750) for 6 hours at 20°C. An HRP-conjugated Goat anti-rabbit IgG polyclonal (1/2500) was used as the secondary antibody.

    See Abreview

References for Anti-TGF beta Receptor I antibody (ab31013)

This product has been referenced in:

See all 13 Publications for this product

Product Wall

You can use any cell line that express endogenous TGF beta receptor 1. Please try any one of HepG2 or A549 cells, both can be used as positive control.

Thank you very much for getting back to me and for confirming the construct details.

If you have a chance, it would be still worth running a good positive control (cytospin preparation from A549 cells or tissue section from placenta) which en...

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Thank you for your enquiry regarding ab31013 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

I would like to reassure you that our Abpromise a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (482N1 murine lung cancer cells)
Loading amount 25000 cells
Specification 482N1 murine lung cancer cells
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 03 2012

Application Western blot
Sample Pig Tissue lysate - whole (liver)
Loading amount 50 µg
Specification liver
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 26 2012

Thanks for your email.

Sorry for the confusion and thank you for bringing this matter to our attention. I have checked and lot # GR138608 does not exist as a lot number for any of our products. It is odd that it was noted on your invoice and ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Mouse mammary gland, MEFs)
Total protein in input 1000 µg
Specification Mouse mammary gland, MEFs
Treatment 2ng/ml TGF-b1 for 30min or 5uM SB431542 for 1hr
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Mar 16 2012

Thank you for your reply.

The only other phospho-smad3 (S423+S425) we have are:

ab11825 - http://www.abcam.com/Smad3-phospho-S423-S425-antibody-ab118825.html

ab52903 - http://www.abcam.com/Smad3-phospho-S423-S425-antibody...

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Thank you for your phone call. DISCOUNT CODE: xxx Expiration date: xxx I am very pleased to hear you would like to accept our offer and test ab31013 in IP. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem thi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"