Anti-TGF beta Receptor I antibody (ab31013)
Overview
- Product nameAnti-TGF beta Receptor I antibodySee all TGF beta Receptor I primary antibodies ...
- DescriptionRabbit polyclonal to TGF beta Receptor I
- Tested applicationsICC/IF, Flow Cyt, IP, WB, IHC-P, IHC-Fr more details
- Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Horse, Chicken, Cow, Pig, Xenopus laevis - Immunogen
Synthetic peptide: VPNEEDPSLDRPFISEGTTLKD, corresponding to Internal sequence amino acids 158-179 of Human TGF beta Receptor I
- Positive controlA549 cells. Placenta.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis antibody has been affinity purified.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab31013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: 1/500. |
| Flow Cyt | Flow Cyt: Use at an assay dependent dilution. |
| IP | IP: Use at 1 µg/mg of lysate. |
| WB | WB: Use at an assay dependent dilution. Predicted molecular weight: 56 kDa. |
| IHC-P | IHC-P: 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Staining requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. |
| IHC-Fr | IHC-Fr: Use at an assay dependent dilution. PubMed: 20223936 |
Target
- FunctionOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta.
- Tissue specificityFound in all tissues examined, most abundant in placenta and least abundant in brain and heart.
- Involvement in diseaseDefects in TGFBR1 are the cause of Loeys-Dietz syndrome type 1A (LDS1A) [MIM:609192]; also known as Furlong syndrome or Loeys-Dietz aortic aneurysm syndrome (LDAS). LDS1 is an aortic aneurysm syndrome with widespread systemic involvement. The disorder is characterized by arterial tortuosity and aneurysms, craniosynostosis, hypertelorism, and bifid uvula or cleft palate. Other findings include exotropy, micrognathia and retrognathia, structural brain abnormalities, intellectual deficit, congenital heart disease, translucent skin, joint hyperlaxity and aneurysm with dissection throughout the arterial tree.
Defects in TGFBR1 are the cause of Loeys-Dietz syndrome type 2A (LDS2A) [MIM:608967]. LDS2 is an aortic aneurysm syndrome with widespread systemic involvement. Physical findings include prominent joint laxity, easy bruising, wide and atrophic scars, velvety and translucent skin with easily visible veins, spontaneous rupture of the spleen or bowel, diffuse arterial aneurysms and dissections, and catastrophic complications of pregnancy, including rupture of the gravid uterus and the arteries, either during pregnancy or in the immediate postpartum period. LDS2 is characterized by the absence of craniofacial abnormalities with the exception of bifid uvula that can be present in some patients.
Defects in TGFBR1 are the cause of aortic aneurysm familial thoracic type 5 (AAT5) [MIM:608967]. Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. - Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain. - Post-translational
modificationsPhosphorylated at basal levels in the absence of ligand binding. Activated by multiple phosphorylation, mainly in the GS region. - Cellular localizationMembrane.
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Database links
- Entrez Gene: 282382 Cow
- Entrez Gene: 7046 Human
- Entrez Gene: 21812 Mouse
- Entrez Gene: 29591 Rat
- Omim: 190181 Human
- SwissProt: O46680 Cow
- SwissProt: P36897 Human
- SwissProt: Q64729 Mouse
- SwissProt: P80204 Rat
- Unigene: 494622 Human
- Unigene: 197552 Mouse
- Unigene: 44402 Rat
see all
Target information above from: UniProt accession
P36897
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- AAT 5 antibodyAAT5 antibodyActivin A receptor type II like kinase 53kDa antibody
- Activin A receptor type II like kinase, 53kD antibodyActivin A receptor type II like protein kinase of 53kD antibodyactivin A receptor type II-like kinase, 53kDa antibodyactivin A receptor type II-like protein kinase of 53kD antibodyActivin receptor like kinase 5 antibodyActivin receptor-like kinase 5 antibodyACVRLK 4 antibodyACVRLK4 antibodyALK 5 antibodyALK-5 antibodyALK5 antibodyLDS1A antibodyLDS2A antibodyMSSE antibodySerine/threonine protein kinase receptor R4 antibodySerine/threonine-protein kinase receptor R4 antibodySKR 4 antibodySKR4 antibodyTbetaR I antibodyTbetaR-I antibodyTGF beta receptor type 1 antibodyTGF beta receptor type I antibodyTGF beta type I receptor antibodyTGF-beta receptor type I antibodyTGF-beta receptor type-1 antibodyTGF-beta type I receptor antibodyTGFBR 1 antibodyTGFBR1 antibodyTGFBR1 protein antibodyTGFR 1 antibodyTGFR-1 antibodyTGFR1 antibodyTGFR1_HUMAN antibodyTransforming growth factor beta receptor 1 antibodyTransforming growth factor beta receptor I (activin A receptor type II like kinase, 53kD) antibodyTransforming growth factor beta receptor I antibodytransforming growth factor, beta receptor 1 antibodytransforming growth factor, beta receptor I (activin A receptor type II-like kinase, 53kD) antibodyTransforming growth factor-beta receptor type I antibody
see all
Anti-TGF beta Receptor I antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TGF beta Receptor I antibody (ab31013)This image is courtesy of an anonymous Abreviewab31013 at 1/50 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed in citrate buffer. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Intense staining of rat heart valve was seen in fresh cut sections compared to weaker staining in those that had been cut 2 weeks previously. Primate placenta was used as a positive control as Abcam recommends - we saw a similar staining pattern to image already on their product page (i.e. clear positive staining was confirmed in the placental outer trophoblast layers). Negative controls (substitution of primary for buffer and suitable isotype) were clear.
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Western blot - TGF beta Receptor I antibody (ab31013)Anti-TGF beta Receptor I antibody (ab31013) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 56 kDa
Observed band size : 56 kDa -
Western blot - TGF beta Receptor I antibody (ab31013)Anti-TGF beta Receptor I antibody (ab31013) at 1/1000 dilution (for 18 hours at 4°C) + Mouse intestine whole tissue lysate at 15 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/8000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 56 kDa
Observed band size : 56 kDa
Exposure time : 1 minuteThis image is courtesy of an anonymous Abreview
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Western blot - TGF beta Receptor I antibody (ab31013)Anti-TGF beta Receptor I antibody (ab31013) at 1/750 dilution (for 16 hours at 4°C) + Rat intestine whole tissue lysate at 20 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 56 kDa
Observed band size : 56 kDa
Exposure time : 2 minutesThis image is courtesy of an anonymous Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TGF beta Receptor I antibody (ab31013)This image is courtesy of an anonymous Abreviewab31013 staining the TGF beta receptor I in Human intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hour at 18°C; antigen retrieval was by heat mediation with citric acid (pH 5.5). Samples were incubated with primary antibody (1/750) for 6 hours at 20°C. An HRP-conjugated Goat anti-rabbit IgG polyclonal (1/2500) was used as the secondary antibody.
References for Anti-TGF beta Receptor I antibody (ab31013)
This product has been referenced in:
- Wang W et al. Inflammasome-independent NLRP3 augments TGF-ß signaling in kidney epithelium. J Immunol 190:1239-49 (2013). Read more (PubMed: 23264657) »
- Chandra M et al. Nuclear translocation of type I transforming growth factor ß receptor confers a novel function in RNA processing. Mol Cell Biol 32:2183-95 (2012). Read more (PubMed: 22473997) »
