You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameAnti-TGF beta 1 antibody [TGFB17]See all TGF beta 1 primary antibodies ...
  • Description
    Mouse monoclonal [TGFB17] to TGF beta 1
  • SpecificityMay crossreact with other members of the TGFB family.
  • Tested applicationsIHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Prokaryotic recombinant protein corresponding to the full length mature transforming growth factor beta 1 molecule.

  • Positive controlPlacenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab49574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/20 - 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for or WB.

    • Target

    • FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
    • Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
    • Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
    • Sequence similaritiesBelongs to the TGF-beta family.
    • Post-translational
      modifications      Glycosylated.
      The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.

      • Target information above from: UniProt accession P01137 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          CED antibodyDPD1 antibodyLAP antibody
          Latency-associated peptide antibodyTGF beta 1 antibodyTGF beta antibodyTGF beta 1 protein antibodyTGF-beta 1 protein antibodyTGF-beta-1 antibodyTGF-beta-5 antibodyTGF-beta1 antibodyTGFB antibodyTgfb-1 antibodyTGFB1 antibodyTGFB1_HUMAN antibodyTGFbeta antibodyTGFbeta1 antibodyTransforming Growth Factor b1 antibodyTransforming Growth Factor beta 1 antibodyTransforming growth factor beta 1a antibodytransforming growth factor beta-1 antibodyTransforming growth factor beta-1 precursor antibodytransforming growth factor, beta 1 antibody
        see all

      Anti-TGF beta 1 antibody [TGFB17] images

      • Immunohistochemistry (Paraffin-embedded sections) - TGF beta 1 antibody [TGFB17] (ab49574)
        Immunohistochemistry (Paraffin-embedded sections) - TGF beta 1 antibody [TGFB17] (ab49574)
        Human breast carcinoma: immunohistochemical staining for Transforming Growth Factor Beta 1 using 1/20 ab49574. Note cytoplasmic staining of tumor cells. Paraffin section.

      References for Anti-TGF beta 1 antibody [TGFB17] (ab49574)

      This product has been referenced in:
      • McCune BK  et al. Localization of transforming growth factor-beta isotypes in lesions of the human breast. Hum Pathol 23:13-20 (1992). Read more (PubMed: 1544664) »

      See 1 Publication for this product

      Publishing research using ab49574 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab49574 Submit a Question for ab49574

      Displaying 1 - 4 of 4 results for Abreviews and Q&A

      10
      Votes: Highest First

      I am concerned about my order. I ordered 100 microliters of ab49574(Anti-TGF beta 1 antibody [TGFB17])and 100 microliters of ab98952(Anti-N Cadherin antibody [5D5]). When they arrived I only had 70 microliters of each. I don't understand why so much was m...

      Read More

      Thank you for contacting us. I sincerely apologize for the shortages and have arranged to send you replacements on order *******. The antibodies you received should still be fine to use, but you will receive the replacements tomorrow. Please let me know i...

      Read More

      Customer called and asked which antibody to select from ab49574, ab27969, ab64715.

      Thank you very much for calling us. It was nice talking to you. As per our conversation we can confirm that all the antibodies are specifically tested in IHC-P with human tissue sections. Abcam guarantee that these antibodies will work in IHC-P. In...

      Read More

      I'd like to know what is the differences between ab49574 and ab1279 monoclonal antibody for TGF beta1.

      Thank you for your enquiry. As there are numerous differences between antibodies ab49574 and ab1279, would it be possible for you to indicate to me specificially what applications you intend to use the antibodies for please? From your previous e-m...

      Read More

      I'd like to know if you have any idea how this antibody work in the pig tissue. I've used monoclonal antibody form [another company] but we cannot make it work. Do you have any suggestion for your product.

      Thank you for your enquiry. As far as we are aware, cross reactivity with pig tissue has not yet been tested for use with ab49574. All species cross-reactivity information is stated on Abcam product datasheets, and these are updated as soon as any new...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"