Overview

  • Product nameAnti-TGN46 antibody
    See all TGN46 primary antibodies
  • Description
    Rabbit polyclonal to TGN46
  • Tested applicationsICC, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:

    KASDYQRLDQKY

    conjugated to KLH by a N-terminal Cysteine residue linker, corresponding to amino acids 426-437 of Human TGN46

  • Positive control
    • A549 cells

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab50595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
WB
IP
ICC/IF
  • Application notesICC/IF: Use at a concentration of 5 - 10 µg/ml.
    IP: Use at 10 - 20 µg.
    WB: Use at a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 80-100 kDa (predicted molecular weight: 47 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay be involved in regulating membrane traffic to and from trans-Golgi network.
    • Tissue specificityIsoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
    • Cellular localizationCell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC14722 antibody
      • TGN 38 antibody
      • TGN 46 antibody
      • TGN 48 antibody
      • TGN 51 antibody
      • TGN38 antibody
      • TGN38 homolog antibody
      • TGN46 antibody
      • TGN48 antibody
      • TGN51 antibody
      • TGOLN 2 antibody
      • TGOLN2 antibody
      • TGON2_HUMAN antibody
      • Trans Golgi network integral membrane protein 2 antibody
      • Trans Golgi network protein (46 48 51kD isoforms) antibody
      • Trans golgi network protein 2 antibody
      • Trans Golgi network protein TGN51 antibody
      • Trans-Golgi network integral membrane protein 2 antibody
      • Trans-Golgi network protein TGN51 antibody
      • TTGN 2 antibody
      • TTGN2 antibody
      see all

    Anti-TGN46 antibody images

    • Human A549 cells were fixed and permeabilized with methanol followed by acetone fixation. Fixed cells were stained with 2.5 µg/ml ab50595. The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate.
    • ab50595 staining human osteosarcoma (143b) cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor® 594 conjugated donkey polyclonal to rabbit diluted 1/500.
    • ab50595 staining TGN46 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde in PBS for 10 minutes, permeabilized with 0.1% Triton X100 and blocked with 2% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 2% BSA + 0.1% Triton X100) for 2 hours at 25°C. An Alexa Fluor® 594-conjugated Goat polyclonal to mouse IgG (dilution 1/1500) was used as secondary antibody.

      See Abreview

    References for Anti-TGN46 antibody (ab50595)

    This product has been referenced in:
    • Ishii Y  et al. Identification of TRAPPC8 as a host factor required for human papillomavirus cell entry. PLoS One 8:e80297 (2013). ICC/IF ; Human . Read more (PubMed: 24244674) »
    • Vagnoni A  et al. Phosphorylation of kinesin light chain 1 at serine 460 modulates binding and trafficking of calsyntenin-1. J Cell Sci 124:1032-42 (2011). Read more (PubMed: 21385839) »

    See all 8 Publications for this product

    Product Wall

    Application Immunocytochemistry
    Sample African Green Monkey Cultured Cells (COS7)
    Specification COS7
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.2% Triton
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Username

    Dr. Yan Qian

    Verified customer

    Submitted Aug 27 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 11 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Human osteosarcoma (HOS) 143b)
    Specification Human osteosarcoma (HOS) 143b
    Fixative Paraformaldehyde
    Permeabilization Yes - 0,1% Triton X-100
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
    Username

    Dr. Alessandro Marcello

    Verified customer

    Submitted Oct 21 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"