Anti-TIA1 antibody [TIA-1] (ab2712)

We found the expiry date given below was not written on the antibody labels. The customers are querying about this and I would like to have some letter mentioning the expiry dates of these two items. Thank you.

ANSWER:

Thank you for your enquiry.

The antibody storage conditions are detailed on every datasheet under the "Storage instructions" section.

We make the general recommend that should our antibodies stipulate storage at -20oC then they can be stored for up to 2 years at this temperature. Likewise we suggest that our antibodies can be stored at -80oC for 10 years. When an antibody can only be kept at 4oC we recommend that it is functional for up to 12 months.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

ReviewID: 4120 AbID: 2712 Status: Submitted

TIA1 antibody [TIA-1] (ab2712):

Sample: Human Cell (HeLa)

Application: Immunocytochemistry/Immunofluorescence

Blocking step:: Serum as blocking agent for 0 hour(s) 15 minute(s)  Concentration: 1%

Fixation:: Paraformaldehyde

Antigen retrieval step:: None

Incubation time:: 1 hour(s)  0 minute(s) 

Positive control:: My samples

Dilution: 1/100

Rating: Didn't work - I would not use it again

Secondary antibody:

Name:: Non-Abcam one was used: [competitor]

Conjugation: Cy3 Dilution:: 1/500

ANSWER:

Thank you for taking the time to submit a review to our system. I am taking your comments seriously and would appreciate your assistance to enable me to look into the source of the problem in more detail. Could you fill out the questionnaire at the following link so I can examine the details of your protocol more closely?

http://www.abcam.com/index.html?section=ihc&pageconfig=technical&intAbID=2712&mode=questionaire

My first recommendation would be to try Anaplastic large cell lymphoma or tonsil as a positive control as stated on our datasheet. I noticed that you fixed with 4% paraformaldehyde, however, we do know with formalin fixation that you need to use antigen retrieval and since this is an intracellular protein that you will need to use a permeabilization. I would recommend permeabilizing with 0.25% Triton X-100 or 100 uM digitonin or 0.5% saponin or trying an alternative fixative such as acetone.

I hope this information helps you and I look forward to receiving your reply.