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Products:Cell Biology >> Apoptosis >> Nucleus >> Other

Anti-TIA1 antibody [TIA-1] (ab2712)

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Overview

Product name

Anti-TIA1 antibody [TIA-1]
See all TIA1 products (5) ...

Description

Mouse monoclonal [TIA-1] to TIA1

Tested applications

IHC-Pmore details

Cross reactivity

Reacts with

Rat, Human, Monkey

Positive control

Anaplastic large cell lymphoma or tonsil.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Primary antibody notes

TIA-1 (T-cell intracytoplasmic antigen) monoclonal antibody reacts with a 15 kDa cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. The expression of TIA-1 was studied in CD30+ anaplastic large cell lymphomas (ALCL), NK cell lymphomas and peripheral T-cell lymphomas and T cell lymphocytosis, B-cell lymphomas and lymphoblastic leukemia, Hodgkin’s etc. Studies showed that 60 to 70% of anaplastic large cell lymphoma reacted with TIA-1. Studies also indicate that TIA-1 reacts with most large granular lymphocytic leukemia’s, hepatosplenic T-cell lymphomas, intestinal T-cell lymphomas, NK-like T-cell lymphomas, NK-cell lymphomas, nasal T/NK-cell lymphomas, subcutaneous T-cell lymphomas, pulmonary angiocentric lymphomas of T or NK phenotype. The author’s concluded from TIA-1 studies that anaplastic large-cell lymphomas are cytotoxic T-or NK-cell neoplasms. All B-cell lymphomas, Hodgkin’s and lymphoblastic leukemias were negative for TIA-1.

Clonality

Monoclonal

Clone number

TIA-1

Myeloma

unknown

Isotype

IgG1

Light chain type

unknown

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Immunology >> Adaptive Immunity >> T Cells >> Non-CD
Cell Biology >> Apoptosis >> Nucleus >> Other

  • Immunocytochemistry/ Immunofluorescence - TIA1 antibody [TIA-1] (ab2712)Immunocytochemistry/ Immunofluorescence - TIA1 antibody [TIA-1] (ab2712) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIA1 antibody [TIA-1] (ab2712)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIA1 antibody [TIA-1] (ab2712) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.

Sequence similarities

Contains 3 RRM (RNA recognition motif) domains.

Cellular localization

Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.

Target information above from: UniProt accessionP31483 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cytotoxic granule associated RNA binding protein 1 antibody
  • Cytotoxic granule associated RNA binding protein antibody
  • mTIA-1 antibody
  • Nucleolysin TIA 1 isoform p40 antibody
  • Nucleolysin TIA-1 isoform p40 antibody
  • Nucleolysin TIA1 isoform p40 antibody
  • p40 TIA 1 antibody
  • p40-TIA-1 antibody
  • RNA binding protein TIA 1 antibody
  • RNA binding protein TIA1 antibody
  • RNA-binding protein TIA-1 antibody
  • T-cell-restricted intracellular antigen-1 antibody
  • TIA 1 antibody
  • TIA 1 cytotoxic granule associated RNA binding protein antibody
  • TIA-1 antibody
  • TIA1 antibody
  • TIA1 cytotoxic granule associated RNA binding protein antibody
  • TIA1 cytotoxic granule associated RNA binding protein like 1 antibody
  • TIA1 protein antibody
  • TIA1_HUMAN antibody
  • TIAL1 antibody
  • TIAR antibody
see all

Database links

Anti-TIA1 antibody [TIA-1] images:

  Immunocytochemistry/ Immunofluorescence - TIA1 antibody [TIA-1] (ab2712)

Immunocytochemistry/ Immunofluorescence - TIA1 antibody [TIA-1] (ab2712)

ab2712 at 1/3300 dilution staining monkey COS 7 cells by ICC/IF. The cells were cultured for 2 hours at 45oC and fixed in cold Methanol: Acetone 1:1 for 10 minutes. The TIA1 antibody was directly conjugated with Alexa Fluor ® 568 and after 1 hour incubation a second fixation step was performed.

This image is courtesy of an Abreview submitted by Mrs Gabriela Elena Oprea

See Abreview

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIA1 antibody [TIA-1] (ab2712)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIA1 antibody [TIA-1] (ab2712)

Human normal spleen. Staining is observed in the cytoplasm. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-TIA1 antibody [TIA-1] (ab2712)

This product has been referenced in:

  • Kim JHet al. The cutaneous lesions of Kikuchi's disease: a comprehensive analysis of 16 cases based on the clinicopathologic, immunohistochemical, and immunofluorescence studies with an emphasis on the differential diagnosis. Hum Pathol 41:1245-54 (2010).Read more (PubMed: 20434191) »
  • Milne Ket al. Tumor-infiltrating T cells correlate with NY-ESO-1-specific autoantibodies in ovarian cancer. PLoS ONE 3:e3409 (2008). IHC-P; Human.Read more (PubMed: 18923710) »

See all 5 publications for this product

Publishing research using ab2712? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"