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Western blot - TIAM1 antibody (ab54458)
(enlarge)
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Product Name
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TIAM1 antibody
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See all TIAM1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TIAM1
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Immunogen
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A synthetic peptide corresponding to amino acids from the C-terminal domain of human TIAM1.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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Recognizes the ~200 kDa TIAM1 protein.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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Jurkat or NIH3T3 cells.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> Regulators
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Relevance
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TIAM1 modulates the activity of RHO like proteins and connects extracellular signals to cytoskeletal activities. It acts as a GDP dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO like GTPases and activates them. It activates RAC1, CDC42, and to a lesser extent RHOA.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7.0-8.0 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TIAM1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TIAM1 antibody images, references, products related to ab54458 and other tools.
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TIAM1 antibody images:
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 Western blot - TIAM1 antibody (ab54458)
All lanes : TIAM1 antibody (ab54458) at 1/1000 dilution
Lane 1 : NIH3T3 whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 25 µg
Predicted band size : 191 kDa
Observed band size : 191 kDa
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Search PubMed (MEDLINE) for references to TIAM1
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TIAM1 antibody - more information
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Customer reviews (feedback) regarding TIAM1 antibody |
Customer FAQs regarding TIAM1 antibody |
Protocols for TIAM1 antibody |
Price and availability of products related to TIAM1 antibody |
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TIAM1 antibody - related products:
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TIAM1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about TIAM1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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