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Immunocytochemistry/ Immunofluorescence - TIAM1 antibody (ab70225)
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Product Name
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TIAM1 antibody
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See all TIAM1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TIAM1
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Immunogen
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Synthetic peptide from a region of Human and Mouse TIAM1 conjugated to an immunogenic carrier protein
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Reacts with
(species key)
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Hu, Ms
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Tested applications
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ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/2000.
IHC-P: 1/300 - 1/3000. Antigen retrieval is recommended.
IHC-Fr: 1/300 - 1/3000.
WB: 1/300 - 1/3000. Predicted molecular weight: 191 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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1 day old mouse cortical cell culture
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> Regulators
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Relevance
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TIAM1 modulates the activity of RHO like proteins and connects extracellular signals to cytoskeletal activities. It acts as a GDP dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO like GTPases and activates them. It activates RAC1, CDC42, and to a lesser extent RHOA.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: Whole serum
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Form
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Lyophilised
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TIAM1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TIAM1 antibody images, references, products related to ab70225 and other tools.
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TIAM1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - TIAM1 antibody (ab70225)
Immunofluorescence analysis of 1 day old mouse cortical cell culture using ab70225 at 1/2000 dilution.
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Search PubMed (MEDLINE) for references to TIAM1
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TIAM1 antibody - more information
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Customer reviews (feedback) regarding TIAM1 antibody |
Customer FAQs regarding TIAM1 antibody |
Protocols for TIAM1 antibody |
Price and availability of products related to TIAM1 antibody |
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TIAM1 antibody - related products:
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TIAM1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about TIAM1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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