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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> Regulators
Overview
Product name
Anti-TIAM1 antibody
See all TIAM1 products (5) ...
Description
Rabbit polyclonal to TIAM1
Tested applications
WB, IHC-Fr, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide from a region of Human and Mouse TIAM1 conjugated to an immunogenic carrier protein
Positive control
1 day old mouse cortical cell culture
Properties
Form
Lyophilised:
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Concentration
Concentration information loading...
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Heterotrimeric G Proteins >> Regulators
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/2000.
IHC-P: 1/300 - 1/3000. Antigen retrieval is recommended.
IHC-Fr: 1/300 - 1/3000.
WB: 1/300 - 1/3000. Predicted molecular weight: 191 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates RAC1, CDC42, and to a lesser extent RHOA.
Tissue specificity
Found in virtually all analyzed tumor cell lines including B- and T-lymphomas, neuroblastomas, melanomas and carcinomas.
Sequence similarities
Belongs to the TIAM family.
Contains 1 DH (DBL-homology) domain.
Contains 1 PDZ (DHR) domain.
Contains 2 PH domains.
Contains 1 RBD (Ras-binding) domain.
Cellular localization
Cell junction. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
Target information above from: UniProt accessionQ13009
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- T cell lymphoma invasion and metastasis 1 antibody
- T lymphoma invasion and metastasis inducing TIAM1 protein antibody
- T-lymphoma invasion and metastasis-inducing protein 1 antibody
see all
Database links
- Entrez Gene: 7074 Human
- Entrez Gene: 21844 Mouse
- Omim: 600687 Human
- SwissProt: Q13009 Human
- SwissProt: Q60610 Mouse
- Unigene: 517228 Human
- Unigene: 310902 Mouse
Anti-TIAM1 antibody images:
Immunocytochemistry/ Immunofluorescence - TIAM1 antibody (ab70225)
Immunofluorescence analysis of 1 day old mouse cortical cell culture using ab70225 at 1/2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIAM1 antibody (ab70225)
IHC image of ab70225 staining in mouse cerebellum formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70225, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-TIAM1 antibody (ab70225)
ab70225 has not yet been referenced specifically in any publications.
Publishing research using ab70225? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"