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Anti-TIAM1 antibody (ab70225)

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Overview

Product name

Anti-TIAM1 antibody
See all TIAM1 products (5) ...

Description

Rabbit polyclonal to TIAM1

Tested applications

WB, IHC-Fr, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Synthetic peptide from a region of Human and Mouse TIAM1 conjugated to an immunogenic carrier protein

Positive control

1 day old mouse cortical cell culture

Properties

Form

Lyophilised:
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole serum

Concentration

Concentration information loading...

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

  • Immunocytochemistry/ Immunofluorescence - TIAM1 antibody (ab70225)Immunocytochemistry/ Immunofluorescence - TIAM1 antibody (ab70225) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIAM1 antibody (ab70225)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIAM1 antibody (ab70225) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab70225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/2000.
IHC-P: 1/300 - 1/3000. Antigen retrieval is recommended.
IHC-Fr: 1/300 - 1/3000.
WB: 1/300 - 1/3000. Predicted molecular weight: 191 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates RAC1, CDC42, and to a lesser extent RHOA.

Tissue specificity

Found in virtually all analyzed tumor cell lines including B- and T-lymphomas, neuroblastomas, melanomas and carcinomas.

Sequence similarities

Belongs to the TIAM family.
Contains 1 DH (DBL-homology) domain.
Contains 1 PDZ (DHR) domain.
Contains 2 PH domains.
Contains 1 RBD (Ras-binding) domain.

Cellular localization

Cell junction. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.

Target information above from: UniProt accessionQ13009 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • T cell lymphoma invasion and metastasis 1 antibody
  • T lymphoma invasion and metastasis inducing TIAM1 protein antibody
  • T-lymphoma invasion and metastasis-inducing protein 1 antibody
  • TIAM-1 antibody
  • TIAM1 antibody
  • TIAM1_HUMAN antibody
see all

Anti-TIAM1 antibody images:

  Immunocytochemistry/ Immunofluorescence - TIAM1 antibody (ab70225)

Immunocytochemistry/ Immunofluorescence - TIAM1 antibody (ab70225)

Immunofluorescence analysis of 1 day old mouse cortical cell culture using ab70225 at 1/2000 dilution.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIAM1 antibody (ab70225)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TIAM1 antibody (ab70225)

IHC image of ab70225 staining in mouse cerebellum formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70225, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-TIAM1 antibody (ab70225)

ab70225 has not yet been referenced specifically in any publications.

Publishing research using ab70225? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"