Anti-TICAM2 antibody (ab17221)
Key features and details
- Rabbit polyclonal to TICAM2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TICAM2 antibody
See all TICAM2 primary antibodies -
Description
Rabbit polyclonal to TICAM2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (Human) (C terminal).
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Positive control
- PC3 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 24 kDa.
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 24 kDa. |
Target
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Function
Functions in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Also involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. -
Tissue specificity
Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas. -
Sequence similarities
Contains 1 TIR domain. -
Domain
The TIR domain mediates the interaction with TRAF6. -
Post-translational
modificationsPhosphorylated by PKCE in response to LPS. Phosphorylation is essential for its function. It is depleted from the membrane upon phosphorylation.
Myristoylated. Required for membrane association which is critical for its ability to initiate efficient signaling. -
Cellular localization
Cytoplasm. Golgi apparatus. Cell membrane. Localized to the plasma membrane as a result of myristoylation. Phosphorylation on Ser-16 leads to its depletion from the membrane. - Information by UniProt
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Database links
- Entrez Gene: 100302736 Human
- Entrez Gene: 353376 Human
- Omim: 608321 Human
- SwissProt: Q86XR7 Human
- Unigene: 709120 Human
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Alternative names
- Putative NF-kappa-B-activating protein 502 antibody
- TCAM2_HUMAN antibody
- TICAM-2 antibody
see all
Images
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Western Blot of PC-3 cell lysate labeling TICAM2 with Anti-TICAM2 antibody (ab17221) at 1µg/ml.
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Anti-TICAM2 antibody (ab17221) at 1 µg/ml + PC-3 cell lysate
Predicted band size: 24 kDa
Western blot using ab17221 at 1µ g/ml on PC-3 cell lysate. -
ab17221 at 10µg/ml staining TICAM2 in Raji cells by ICC/IF
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab17221 has been referenced in 2 publications.
- Alsaqer SF et al. Inhibition of LSD1 epigenetically attenuates oral cancer growth and metastasis. Oncotarget 8:73372-73386 (2017). PubMed: 29088714
- Endo Y et al. Differences in PGE2 production between primary human monocytes and differentiated macrophages: role of IL-1ß and TRIF/IRF3. PLoS One 9:e98517 (2014). WB ; Human . PubMed: 24870145