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Overview

  • Product nameTIE1 Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 0.6 ng/ml
  • Range
    0.68 ng/ml - 500 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 105.9 93% - 115%
    Serum 97 86% - 107%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100649, a Human TIE1 (Tyrosine Kinase with Ig and EGFhomology domain 1) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TIE1 in serum (plasma is not recommended), cell culture supernatants. This assay employs an antibody specific for human TIE1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIE1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TIE1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIE1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • RelevanceTIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.
    • Alternative names
        EC 2.7.10.1JTK14receptor tyrosine kinase
        soluble TIE1 variant 1soluble TIE1 variant 2soluble TIE1 variant 3soluble TIE1 variant 4soluble TIE1 variant 5TIEtyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1tyrosine kinase with immunoglobulin like and EGF like domains 1tyrosine-protein kinase receptor Tie 1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100649 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      TIE1 Human ELISA Kit images

      • ELISA - TIE1 Human ELISA Kit (ab100649)
        ELISA - TIE1 Human ELISA Kit (ab100649)

      Protocols

      References for TIE1 Human ELISA Kit (ab100649)

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