TIE1 Human ELISA Kit (ab100649)
- Product nameTIE1 Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 0.6 ng/ml
- Range0.68 ng/ml - 500 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 105.9 93% - 115% Serum 97 86% - 107%
- Species reactivityReacts with: Human
- Product overview
ab100649, a Human TIE1 (Tyrosine Kinase with Ig and EGFhomology domain 1) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TIE1 in serum (plasma is not recommended), cell culture supernatants. This assay employs an antibody specific for human TIE1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIE1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TIE1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIE1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 500X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent A diluent buffer . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody Tie-1: biotinylated anti-human Tie-1 2 vials Stop Solution Item I 1 x 25ml TIE 1 Microplate (12 strips x 8 wells) coated with anti-human Tie-1 1 unit TIE 1 Standard Human (recombinant) 2 vials TMB One-Step Substrate Reagent Item H 1 x 12ml
- EC 126.96.36.199JTK14receptor tyrosine kinase
- soluble TIE1 variant 1soluble TIE1 variant 2soluble TIE1 variant 3soluble TIE1 variant 4soluble TIE1 variant 5TIEtyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1tyrosine kinase with immunoglobulin like and EGF like domains 1tyrosine-protein kinase receptor Tie 1
- SwissProt: P35590 Human
Our Abpromise guarantee covers the use of ab100649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for TIE1 Human ELISA Kit (ab100649)
ab100649 has not yet been referenced specifically in any publications.