Anti-TIE2 antibody [TEK4] (Phycoerythrin) (ab95722)
- Product nameAnti-TIE2 antibody [TEK4] (Phycoerythrin)See all TIE2 primary antibodies ...
- DescriptionRat monoclonal [TEK4] to TIE2 (Phycoerythrin)
- Tested applicationsFlow Cyt more details
- Species reactivityReacts with: Mouse
Mouse TIE2 extracellular domain
- Positive controlMouse bone marrow cell suspensions
- Storage instructionsStore at +4°C in the dark. Do not freeze.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS, 150mM Sodium chloride, pH 7.2
- Concentration information loading...
- PurityProtein G purified
- Clonality Monoclonal
- Clone numberTEK4
- Light chain typekappa
Our Abpromise guarantee covers the use of ab95722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Flow Cyt: Use 1-2µg for 105-8 cells. A final volume of 100µl is recommended.|
- FunctionThis protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.
- Tissue specificityPredominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
- Involvement in diseaseDefects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.
- Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
Contains 3 EGF-like domains.
Contains 3 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
modificationsPhosphorylated. Phosphorylation is induced by ANGPT1 and ANGPT2. ANGPT1-induced phosphorylation is impaired during hypoxia. Dephosphorylated by PTPRB.
Autophosphorylation at Tyr-1108 is critical for coupling downstream cell migration signal transduction pathways with ANG1 stimulation in endothelial cells.
- Cellular localizationMembrane.
- Angiopoietin 1 receptor antibodyAngiopoietin-1 receptor antibodyCD202b antibody
- CD202b antigen antibodyEndothelial tyrosine kinase antibodyEndothelium specific receptor tyrosine kinase 2 antibodyhTIE 2 antibodyhTIE2 antibodyhTIE2 antibodyHyk antibodyp140 TEK antibodyp140 TEK antibodySoluble TIE2 variant 1 antibodySoluble TIE2 variant 2 antibodyTEK antibodytek tyrosine kinase antibodyTEK tyrosine kinase endothelial antibodytek tyrosine kinase, endothelial antibodyTIE 2 antibodyTIE2_HUMAN antibodyTunica interna endothelial cell kinase antibodyTyrosine kinase with Ig and EGF homology domains 2 antibodyTyrosine protein kinase receptor TEK antibodyTyrosine protein kinase receptor TIE 2 antibodyTyrosine-protein kinase receptor TEK antibodyTyrosine-protein kinase receptor TIE-2 antibodyVenous malformations multiple cutaneous and mucosal antibodyVMCM 1 antibodyVMCM antibodyVMCM1 antibody
Anti-TIE2 antibody [TEK4] (Phycoerythrin) images
Staining of Mouse bone marrow with Anti-Mouse Ly-6A/E (Sca-1) APC, Anti-Mouse CD117 (c-Kit) APC and 1.0 µg of ab95722. Total viable lineage-negative cells were used for analysis.
References for Anti-TIE2 antibody [TEK4] (Phycoerythrin) (ab95722)
This product has been referenced in:
- Lelievre E et al. Deficiency in the p110alpha subunit of PI3K results in diminished Tie2 expression and Tie2(-/-)-like vascular defects in mice. Blood 105:3935-8 (2005). Read more (PubMed: 15687236) »
- Hsu HC et al. Hematopoietic stem cells express Tie-2 receptor in the murine fetal liver. Blood 96:3757-62 (2000). Read more (PubMed: 11090057) »