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Anti-TIE2 antibody [TEK4] (Phycoerythrin) (ab95722)

Submit an Abreview Q&A (2)Specific References (2)

Overview

  • Product nameAnti-TIE2 antibody [TEK4] (Phycoerythrin)See all TIE2 primary antibodies ...
  • Description
    Rat monoclonal [TEK4] to TIE2 (Phycoerythrin)
  • ConjugationPhycoerythrin
  • Tested applicationsFlow Cyt more details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse TIE2 extracellular domain

  • Positive controlMouse bone marrow cell suspensions

Properties

Applications

Our Abpromise guarantee covers the use of ab95722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Flow Cyt: Use 1-2µg for 105-8 cells. A final volume of 100µl is recommended.

Target

  • FunctionThis protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.
  • Tissue specificityPredominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
  • Involvement in diseaseDefects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
    Contains 3 EGF-like domains.
    Contains 3 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Phosphorylated. Phosphorylation is induced by ANGPT1 and ANGPT2. ANGPT1-induced phosphorylation is impaired during hypoxia. Dephosphorylated by PTPRB.
    Autophosphorylation at Tyr-1108 is critical for coupling downstream cell migration signal transduction pathways with ANG1 stimulation in endothelial cells.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession Q02763 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Angiopoietin 1 receptor antibodyAngiopoietin-1 receptor antibodyCD202b antibody
        CD202b antigen antibodyEndothelial tyrosine kinase antibodyEndothelium specific receptor tyrosine kinase 2 antibodyhTIE 2 antibodyhTIE2 antibodyhTIE2 antibodyHyk antibodyp140 TEK antibodyp140 TEK antibodySoluble TIE2 variant 1 antibodySoluble TIE2 variant 2 antibodyTEK antibodytek tyrosine kinase antibodyTEK tyrosine kinase endothelial antibodytek tyrosine kinase, endothelial antibodyTIE 2 antibodyTIE2_HUMAN antibodyTunica interna endothelial cell kinase antibodyTyrosine kinase with Ig and EGF homology domains 2 antibodyTyrosine protein kinase receptor TEK antibodyTyrosine protein kinase receptor TIE 2 antibodyTyrosine-protein kinase receptor TEK antibodyTyrosine-protein kinase receptor TIE-2 antibodyVenous malformations multiple cutaneous and mucosal antibodyVMCM 1 antibodyVMCM antibodyVMCM1 antibody
      see all

    Anti-TIE2 antibody [TEK4] (Phycoerythrin) images

    • Flow Cytometry - TIE2 antibody [TEK4] (Phycoerythrin) (ab95722)
      Flow Cytometry - TIE2 antibody [TEK4] (Phycoerythrin) (ab95722)
      Staining of Mouse bone marrow with Anti-Mouse Ly-6A/E (Sca-1) APC, Anti-Mouse CD117 (c-Kit) APC and 1.0 µg of ab95722. Total viable lineage-negative cells were used for analysis.

    References for Anti-TIE2 antibody [TEK4] (Phycoerythrin) (ab95722)

    This product has been referenced in:
    • Lelievre E  et al. Deficiency in the p110alpha subunit of PI3K results in diminished Tie2 expression and Tie2(-/-)-like vascular defects in mice. Blood 105:3935-8 (2005). Read more (PubMed: 15687236) »
    • Hsu HC  et al. Hematopoietic stem cells express Tie-2 receptor in the murine fetal liver. Blood 96:3757-62 (2000). Read more (PubMed: 11090057) »

    See all 2 Publications for this product

    Publishing research using ab95722 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab95722 Submit a Question for ab95722

    Displaying 1 - 2 of 2 results for Abreviews and Q&A

    10
    Votes: Highest First

    Hopefully this will still work for my cells which may have a higher signal then the one shown. I think it would be nicer to advertise a more optimal result along side this TDS for Tie2.   

    Thank you for your response. I do agree.

    One way in which we are able to get images for the datasheets is from customer reviews of our products. Should you decide that ab95722 is the correct product for your experiments,I would like to encourag...

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    Customer kindly called to enquire about the image on the DS for ab95722.

    1) This image does not show/mention the associated antibody
    2) This image does not show any TIE2 positivity in the FL2 channel, where PE would be expected. 

    Read More

    Thank you for contacting us.

    I did contact the lab regarding this question for you. I asked that while wedo see the APC conjugated c-Kit positive cells we were having difficulty reconciling this with the apparent low levels of PE. Shouldn't t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"