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Anti-TIE2 (phospho Y1108) antibody (ab63346)

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Overview

  • Product nameAnti-TIE2 (phospho Y1108) antibodySee all TIE2 primary antibodies ...
  • Description
    Rabbit polyclonal to TIE2 (phospho Y1108)
  • Specificityab63346 detects endogenous levels of TIE2 only when phosphorylated at tyrosine 1108.
  • Tested applicationsELISA, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized phosphopeptide derived from human TIE2 around the phosphorylation site of tyrosine 1108 (TLYPEK).

  • Positive controlHepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab63346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: 1/10000.
ICC/IF ICC/IF: 1/500 - 1/1000.

Target

  • FunctionThis protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.
  • Tissue specificityPredominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
  • Involvement in diseaseDefects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
    Contains 3 EGF-like domains.
    Contains 3 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Phosphorylated. Phosphorylation is induced by ANGPT1 and ANGPT2. ANGPT1-induced phosphorylation is impaired during hypoxia. Dephosphorylated by PTPRB.
    Autophosphorylation at Tyr-1108 is critical for coupling downstream cell migration signal transduction pathways with ANG1 stimulation in endothelial cells.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession Q02763 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Angiopoietin 1 receptor antibodyAngiopoietin-1 receptor antibodyCD202b antibody
        CD202b antigen antibodyEndothelial tyrosine kinase antibodyEndothelium specific receptor tyrosine kinase 2 antibodyhTIE 2 antibodyhTIE2 antibodyhTIE2 antibodyHyk antibodyp140 TEK antibodyp140 TEK antibodySoluble TIE2 variant 1 antibodySoluble TIE2 variant 2 antibodyTek antibodytek tyrosine kinase antibodyTEK tyrosine kinase endothelial antibodytek tyrosine kinase, endothelial antibodyTIE 2 antibodyTIE2_HUMAN antibodyTunica interna endothelial cell kinase antibodyTyrosine kinase with Ig and EGF homology domains 2 antibodyTyrosine protein kinase receptor TEK antibodyTyrosine protein kinase receptor TIE 2 antibodyTyrosine-protein kinase receptor TEK antibodyTyrosine-protein kinase receptor TIE-2 antibodyVenous malformations multiple cutaneous and mucosal antibodyVMCM 1 antibodyVMCM antibodyVMCM1 antibody
      see all

    Anti-TIE2 (phospho Y1108) antibody images

    • Immunocytochemistry/ Immunofluorescence - TIE2 (phospho Y1108) antibody (ab63346)
      Immunocytochemistry/ Immunofluorescence - TIE2 (phospho Y1108) antibody (ab63346)
      Immunofluorescence analysis of TIE2 (phospho Y1108) in HepG2 cells, using 1/500 ab63346. Left: untreated sample. Right: sample treated with immunising phosphopeptide.

    References for Anti-TIE2 (phospho Y1108) antibody (ab63346)

    ab63346 has not yet been referenced specifically in any publications.

    Publishing research using ab63346 ? Please let us know so that we can cite the reference in this datasheet

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    Replacement for ab63346 is not working in the same way the first vial was not working, even though it is a different lot. The antibody still specifically stains the nucleus but the target protein is only found at the cell periphery.

    I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To...

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