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All lanes : Anti-TIM 1 antibody (ab47068) at 1 µg/ml
Lane 1 : Human brain lysate with no immunizing peptide
Lane 2 : Human brain lysate with immunizing peptide
Lane 3 : Mouse brain lysate
Predicted band size : 39 kDa
Observed band size : 21 kDa (why is the actual band size different from the predicted?)
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