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Products:Immunology >> Adaptive Immunity >> T Cells >> Non-CD
Overview
Product name
Anti-TIM 1 antibody
See all TIM 1 products (9) ...
Description
Mouse monoclonal to TIM 1
Tested applications
WB, ELISA, Flow Cytmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chimpanzee
Immunogen
Recombinant fragment with tag: KVGGEAGPSV TLPCHYSGAV TSMCWNRGSC SLFTCQNGIV WTNGTHVTYR KDTRYKLLGD LSRRDVSLTI ENTAVSDSGV YCCRVEHRGW FNDMKITVSL, corresponding to amino acids 23-123 of Human TIM 1
KVGGEAGPSV TLPCHYSGAV TSMCWNRGSC SLFTCQNGIV WTNGTHVTYR KDTRYKLLGD LSRRDVSLTI ENTAVSDSGV YCCRVEHRGW FNDMKITVSL
Positive control
TIM 1 transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Research areas
Immunology >> Immune System Diseases >> Allergens
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Immunology >> Adaptive Immunity >> T Cells >> Non-CD
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Western blot - TIM 1 antibody (ab66062)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 36 kDa (predicted molecular weight: 39 kDa).
ELISA: Use at an assay dependent dilution. (The detection limit for recombinant tagged TIM 1 is approximately 10ng/ml if used as a capture antibody.)
Flow Cyt: Use at an assay dependent dilution. (See Abreview.)
Target
Function
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
Tissue specificity
Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
Sequence similarities
Belongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ96D42
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HAVCR 1 antibody
- HAVcr-1 antibody
- HAVCR1 antibody
see all
Database links
- Entrez Gene: 26762 Human
- Omim: 606518 Human
- SwissProt: Q96D42 Human
- Unigene: 129711 Human
Anti-TIM 1 antibody images:
Western blot - TIM 1 antibody (ab66062)
All lanes : Anti-TIM 1 antibody (ab66062) at 1 µg/ml
Lane 1 : TIM 1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size : 39 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
References for Anti-TIM 1 antibody (ab66062)
ab66062 has not yet been referenced specifically in any publications.
Publishing research using ab66062? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"